| 受精卵原核显微穿刺导入DNA制备转基因兔 |
| |
| 引用本文： | 曹西南,熊亚隆,纳冬荃. 受精卵原核显微穿刺导入DNA制备转基因兔[J]. 昆明医学院学报, 2005, 26(4): 49-54 |
| |
| 作者姓名： | 曹西南 熊亚隆 纳冬荃 |
| |
| 作者单位： | 1. 昆明医学院基础医学院分子生物学实验室,昆明,650031
2. 昆明医学院基础医学院公共卫生学院劳动卫生教研室,昆明,650031 |
| |
| 基金项目： | 昆明市科委研究基金（昆科工字2001-13） |
| |
| 摘 要： | 目的：受精卵原核显微DNA注射是转基因动物制备的最常用、最可靠的方法．研究技术上更为简易的受精卵原核显微穿刺导入DNA制备转基因兔的可行性．方法：重组转基因载体pCA-CHA,采用细胞原核显微穿刺将DNA导入兔受精卵制备转基因兔，用PCR和Southern Blot方法鉴定转基因兔．结果：转基因处理后获得仔兔92只，以PCR检测结果计算，转基因总效率为1．63%～2．40％，整合率为11．54%～16．13％；以Southern Blot检测结果计算，转基因总效率为0．27％，整合率为1．92％．结论：受精卵原核显微穿刺导入DNA与受精卵原核显微DNA注射制备转基因兔的结果相当．
|
| 关 键 词： | 细胞核显微穿刺 转基因 兔 |
| 文章编号： | 1003-4706(2005)04-0049-06 |
| 收稿时间： | 2005-09-12 |
| 修稿时间： | 2005-09-12 |
Introducing DNA into Zygotes through Nuclear Micro- Pricking to Produce Transgenic Rabbit |
| |
| CAO Xi-nan,XIONG Ya-long,NA Dong-quan. Introducing DNA into Zygotes through Nuclear Micro- Pricking to Produce Transgenic Rabbit[J]. Journal of Kunming Medical College, 2005, 26(4): 49-54 |
| |
| Authors: | CAO Xi-nan XIONG Ya-long NA Dong-quan |
| |
| Affiliation: | 1. Laboratory of Molecular Biology, School of Basic Medicine, Kunming Medical College ; 2. Dept. of Occupational Health, School of Public Health, Kunming Medical College, Kunming 650031, China |
| |
| Abstract: | Objective:Nuclear DNA micro-injection of zygotes is most common and most reliable method for transgenic mammalian production.We have successfully produced transgenic rabbits by nuclear micro-pricking to introduce DNA into zygotes(a more simple and easy possessed method).Methods:Expression vector pCA-CHA was constructed and used for producing transgenic rabbits by nuclear DNA micro-pricking of zygotes.Results:PCR and Southern blotting were applied for identification of transgenic rabbits.The total efficiency of the gene transfer was between 0.96% and 1.63% with integration rates of 6.45% and 11.54% by PCR analysis.The total efficiency of the gene transfer was 0.27% with an integration rate of 1.92% determined by Southern blotting.Conclusion:These results are compatible with ones obtained by nuclear DNA micro-injection of rabbit zygotes. |
| |
| Keywords: | Nuclear micro-pricking Transgene Rabbit |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
