黄岑属植物化学研究——Ⅳ.薄叶黄芩中葡萄糖醛酸黄酮甙的高效液相色谱(英文)

在薄叶黄芩成分分离的过程中,用反相HPLC分离葡萄糖醛酸黄酮甙,包括柱和溶媒系统等的条件选择:并发现当羟基和葡萄糖醛酸基的取代位置不同时,其保留时间的变化规律(简称构保关系)。例如,当羟基从4位变化到2位时,保留时间增大:2′,6,8位羟基的存在,保留时间依次增大;而当葡萄糖醛酸基从7位变化到8位时,保留时间相应变小。有人提出在相同位置上都存在有羟基的黄酮类化合物在一起时,是用HPLC不能得到很好分离的“临界群”,如易与羰基形成内氢键的5位羟基。本文方法解决了这一问题,成功地分离到的7个葡萄糖醛酸黄酮甙,5位都存在有羟基。本文还介绍了两种不同型号的ODS柱对分离葡萄糖醛酸黄酮甙的效果。

Studies on the constituents of Scutellaria species. IV. High performance liquid chromatography of glucuronylflavonoids in Scutellaria ikonnikovii juz

Recently several procedures have been reported for the simultaneous separation of flavonoid glycosides by high performance liquid chromatography (HPLC), and reversed phase column is more suitable than normal phase column. Even so, the simultaneous separation of flavonoids with closely related structures is not easy. We investigated a successful procedure for the simultaneous separation of glucuronyl flavonoids with 5-hydroxyl group by using a reversed phase column combined with gradient elution in the constituent studies of Scutellaria ikonnikovii Juz.. We have also studied the relationship between the retention time and the position of hydroxyls and glucuronyls in the chemical structure.