| RP-HPLC法测定人血清中拉莫三嗪的浓度 |
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| 引用本文： | 张国阳. RP-HPLC法测定人血清中拉莫三嗪的浓度[J]. 中国药房, 2010, 0(34): 3206-3208 |
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| 作者姓名： | 张国阳 |
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| 作者单位： | 江苏淮安市第三人民医院,淮安市223001 |
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| 摘 要： | 目的:建立以反相高效液相色谱法测定人血清中拉莫三嗪浓度的方法。方法:色谱柱为KromasilC18,流动相为甲醇∶乙腈∶0.03mo·lL-1醋酸铵(乙酸调pH值至3.5±0.1)=12∶12∶27;流速为1.0mL·min-1,柱温为室温,检测波长为310nm,进样量为20μL。结果:拉莫三嗪血药浓度在0.5～30.0mg·L-1范围内线性关系良好(r=0.9986);方法回收率为99.00%～102.00%;日内、日间RSD分别为1.66%～2.50%、2.18%～2.98%。结论:本方法操作简便、快速、灵敏、重复性好、回收率及精密度高,可用于拉莫三嗪的治疗药物监测。
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| 关 键 词： | 反相高效液相色谱法 拉莫三嗪 血药浓度 药动学 |
Content Determination of Lamotrigine in Human Serum by RP-HPLC |
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| ZHANG Guo-yang. Content Determination of Lamotrigine in Human Serum by RP-HPLC[J]. China Pharmacy, 2010, 0(34): 3206-3208 |
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| Authors: | ZHANG Guo-yang |
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| Affiliation: | ZHANG Guo-yang(Huai＇an Municipal Third People＇s Hospital of Jiangsu Province,Huai＇an 223001,China) |
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| Abstract: | OBJECTIVE：To develop an RP-HPLC method for the content determination of lamotrigine in human serum.METHODS：HPLC method was conducted on Kromasil C18（250 mm×4.6 mm,5 μm）column with the mobile phase consisted of methanol-acetonitrile-0.03 mol·L-1 NH4AC （pH value adjusted to 3.5±0.1 with acetic acid）=12：12：27.The flow rate was set at 1.0 mL·min-1 and the detection wavelength were at 310 nm.The column temperature was room temperature and injection volume was 20 μL.RESULTS：The linear range of lamotrigine were 0.5～30.0 mg·L-1 （r=0.998 6） with recovery of 99.00%～102.00%.The RSD of intra-day and inter-day were 1.66%～2.50%,2.18%～2.98% respectively.CONCLUSION：The method is simple,rapid,sensitive and reproducible with high recovery and good precision for pharmaceutical monitoring of lamotrigine. |
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| Keywords: | RP-HPLC Lamotrigine Serum concentration Pharmacokinetics |
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