HPLC法同时测定夏枯草中齐墩果酸和熊果酸的含量

目的:建立以β-环糊精作流动相添加剂的高效液相色谱法同时测定夏枯草中齐墩果酸和熊果酸含量的方法。方法:以夏枯草为样品,色谱柱为Tigerkin ODS3(250mm×4.6mm,5μm),柱温为40℃,流动相为甲醇-10mmo·lL-1β-环糊精1%醋酸胺水溶液(86:14),流速为1.0mL·min-1,检测波长为210nm。结果:齐墩果酸、熊果酸进样量分别在0.5～5.0μg(r=0.9992)、1.0～10.0μg(r=0.9998)范围内与各自峰面积积分值呈良好线性关系。二者的高、中、低平均回收率分别为97.3%(RSD=1.04%)、98.0%(RSD=0.87%)、101.6%(RSD=1.65%),97.8%(RSD=1.17%)、98.7%(RSD=0.93%)、100.4%(RSD=1.72%)。结论:本方法简便、准确、重复性好,可用于夏枯草的质量控制,并可作为同时测定齐墩果酸和熊果酸的新方法。

Content Determination of Oleanolic Acid and Ursolic Acid in Prunella vulgaris by HPLC

OBJECTIVE：To establish HPLC method for the content determination of oleanolic acid and ursolic acid in Prunella vulgris with β-CD as additive agent of mobile phase.METHODS：The sample was Prunella vulgaris.The separation was performed on a Tigerkin ODS3（250 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-10 mmol·L^-1 β-CD 1% ammonium acetate（86：14） at flow rate of 1.0 mL·min^-1.The column temperature was set at 40 ℃ and UV detection wavelength was 210 nm.RESULTS：The linear range of oleanolic acid and ursolic acid were 0.5～5.0 μg（r=0.999 2） and 1.0～10.0 μg（r=0.999 8） respectively.High,medium and low average recovery of oleanolic acid were 97.3%（RSD=1.04%）,98.0%（RSD=0.87%）,101.6%（RSD=1.65%）,respectively.Those of ursolic acid were 97.8%（RSD=1.17%）,98.7%（RSD=0.93%）,100.4%（RSD=1.72%）,respectively.CONCLUSION：The new method is simple,accurate and reproducible for the quality control of P.vulgaris and simultaneous determination of oleanolic acid and ursolic acid.