| HPLC法测定不同来源甘草中甘草酸的含量 |
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| 引用本文： | 唐丽琴,张善堂,刘圣,石允卉. HPLC法测定不同来源甘草中甘草酸的含量[J]. 中国药房, 2010, 0(39): 3700-3701 |
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| 作者姓名： | 唐丽琴 张善堂 刘圣 石允卉 |
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| 作者单位： | 安徽医科大学附属省立医院药剂科,合肥市230001 |
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| 基金项目： | 安徽省自然科学基金面上项目（090413106） |
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| 摘 要： | 目的:建立以高效液相色谱法测定甘草中有效成分甘草酸含量的方法,并测定不同产地和基原甘草药材中甘草酸的含量。方法:色谱柱为Hypersil-C18(250mm×4.6mm,5μm),流动相为甲醇-0.2mo·lL-1醋酸铵溶液-冰醋酸-三乙胺(64:36:1.5:0.02),流速为1.0mL·min-1,检测波长为252nm,柱温为30℃。结果:甘草酸检测浓度在0.0416～0.2080mg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为101.3%,RSD=1.22%(n=9)。结论:不同来源甘草药材中甘草酸的含量差异较大,野生甘草中甘草酸的含量高于家种。
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| 关 键 词： | 甘草 甘草酸 高效液相色谱法 含量 来源 |
Content Determination of Glycyrrhizic Acid in Glycyrrhiza uralensis from Different Sources by HPLC |
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| TANG Li-qin,ZHANG Shan-tang,LIU Sheng,SHI Yun-hui. Content Determination of Glycyrrhizic Acid in Glycyrrhiza uralensis from Different Sources by HPLC[J]. China Pharmacy, 2010, 0(39): 3700-3701 |
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| Authors: | TANG Li-qin ZHANG Shan-tang LIU Sheng SHI Yun-hui |
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| Affiliation: | (Dept.of Pharmacy,The Affiliated Anhui Provincial Hospital,Anhui Medical University,Hefei 230001,China) |
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| Abstract: | OBJECTIVE：To establish the HPLC method for the content determination of glycyrrhizic acid in Glycyrrhiza uralensis from different sources.METHODS：The separation was performed on Hypersil-C18（250 mm×4.6 mm,5 μm）column with mobile phase consisted of methanol-0.2 mol·L^-1 ammonium acetate-glacial acetic acid-triethylamine（64：36：1.5：0.02） with the flow rate of 1.0 mL·min-1.The detection wavelength was set at 252 nm and column temperature was 30 ℃.RESULTS：The linear range of glycyrrhizic acid was 0.041 6～0.208 0 mg·mL^-1（r=0.999 9） with an average recovery of 101.3%（RSD=1.22%,n=9）.CONCLUSION：The content of glycyrrhizic acid is variant in different origin and sources.The content of glycyrrhizic acid in wild G.uralensis is higher than that of domestic species. |
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| Keywords: | Glycyrrhiza uralensis Glycyrrhizic acid HPLC Content Source |
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