RP-HPLC法测定射干合剂中黄酮类多指标成分的含量

目的:建立以反相高效液相色谱法同时测定射干合剂中黄芩苷等5种黄酮类化合物含量的方法。方法:样品经超声提取后,用InertsilODS-3色谱柱分离样品,流动相为乙腈-1‰磷酸(pH2.25,梯度洗脱),流速为1mL·min-1,柱温为室温,检测波长为260nm;外标法定量。结果:黄芩苷、黄芩素、汉黄芩素、射干苷、次野鸢尾黄素的线性范围均为0.05～50μg·mL-1(r均≥0.9989);平均加样回收率为96.52%～101.22%,RSD均≤4.4%(n=9)。结论:本方法简便、准确、灵敏度高,可用于射干合剂中黄酮类化合物多指标成分的含量测定,也可用于其制剂及体内指标成分的研究。

Content Determination of Multiple Indicator Constituents of Flavonoids in Belamcanda chinensis Mixture by RP-HPLC

OBJECTIVE：To establish RP-HPLC method for the content determination of 5 kinds of multiple indicator constituents of flavonoids in Belamcanda chinensis mixture such as baicalin.METHODS：Samples were extracted with selective ultrasound extraction.The separation was performed on InertsilODS-3 column with mobile phase consisted of acetonitrile-1‰ phosphoric acid（pH=2.25,gradient elution） at flow rate of 1 mL·min^-1.The column temperature was room temperature and detection wavelength was set at 260 nm.The method was qualified by an external standard mode.RESULTS：The linear ranges of baicalin,baicalein,wogonin,tectoridin and irisflorentin were all 0.05～50 μg·mL-1（r≥0.998 9） with average recoveries of 96.52%～101.22%（RSD≤4.4%,n=9）.CONCLUSION：This method is simple,convenient,accurate and sensitive for content determination of multiple indicator constituents of flavonoids in B.chinensis mixture and constituent study of preparation and internal index constituent.