气相色谱法测定保济丸中百秋李醇的含量

目的:建立以气相色谱法测定保济丸中百秋李醇含量的方法,制定并完善保济丸的质量标准。方法:采用非极性毛细管柱AT.SE-54(15m×0.25mm×0.33μm),柱温采用程序升温:起始150℃,保持23min,以8℃·min-1速率升至230℃,保持2min,进样口温度为280℃,检测器(FID)温度为280℃,载气为氮气,流速为1.0mL·min-1。结果:百秋李醇进样量在0.12～0.36μg范围内线性关系良好(r=0.9999),平均加样回收率为98.62%,RSD=4.69%。结论:该方法稳定、结果可靠、重复性好,可作为保济丸质量控制标准之一。

Content Determination of Patchouli Alcohol in Baoji Pills by Gas Chromatography

OBJECTIVE：To develop gas chromatography （GC） method for the content determination of patchouli alcohol in Baoji pills and to establish the quality standard of it.METHODS：The determination was performed on AT.SE-54 capiltary column （15 m×0.25 mm×0.33 μm）.The increase of column temperature was controlled by programming; the initial temperature was 150 ℃ and maintained for 23 min, and then rose at 8 ℃·min-1 up to 230 ℃ for 2 min.The injector temperature and detector temperature were both 280 ℃.Nitrogen was used as carrier and the flow rate was 1.0 mL·min-1.RESULTS：The linear ranges of patchouli alcohol was 0.12～0.36 μg （r=0.999 9） with an average recovery of 98.62% （RSD=4.69%）.CONCLUSION：The method is accurate, reliable and reproducible, and it can be used for the quality control of Baoji pills.