正交试验优选双水相分配与超声提取集成法提取分离连钱草总黄酮的工艺

目的:优选双水相分配与超声提取集成法提取分离连钱草总黄酮的最佳工艺。方法:以超声提取时间、丙醇体积分数、料液比、(NH4)2SO4浓度为考察因素,连钱草总黄酮提取率为考察指标,通过正交试验优选其最佳提取工艺条件。结果:本法最佳提取工艺条件为超声提取时间30min,丙醇体积分数为50%,料液比1:30,(NH4)2SO4用量为30%。结论:本法操作简便、条件温和、效率高,具有较大优势。

Study on Optimization of Extraction Technology of Flavonoids from Glechoma longituba by the Integration of Aqueous Two-Phase Partitioning with Ultrasonic Extraction

OBJECTIVE：To optimize extraction technology of flavonoids from Glechoma longituba by aqueous two-phase partitioning with ultrasonic extraction.METHODS：The extraction technology of flavonoids from G.longituba were optimized by orthogonal experiment with ultrasonic extraction time,the volume fraction of propanol,the ratio of solid to solution,amount of（NH4）2SO4 as factors and with extraction rate of flavonoids from G.longituba as index.RESULTS：The optimal conditions were as follows：extracting for 30 min with ultrasonic wave,the volume fraction of propanol 50%,the ratio of solid to solution 1：30,and the amount of（NH4）2SO4 30%.CONCLUSION：Established extraction technology which is characterized with simple operation and mild conditions shows great advantage.