| HPLC法测定小儿止咳颗粒中黄芩苷的含量 |
| |
| 引用本文： | 廖伟明,刘桂珍,周国波. HPLC法测定小儿止咳颗粒中黄芩苷的含量[J]. 中国药房, 2010, 0(31): 2946-2947 |
| |
| 作者姓名： | 廖伟明 刘桂珍 周国波 |
| |
| 作者单位： | 深圳市宝安区人民医院,深圳市518000 |
| |
| 摘 要： | 目的:建立以高效液相色谱法测定小儿止咳颗粒中黄芩苷含量的方法。方法:色谱柱为YWGC18(150mm×4.6mm,μm),10流动相为甲醇-水-磷酸(47∶53∶0.2),流速为2.5mL·min-1,检测波长为280nm,进样量为10μL。结果:黄芩苷的检测浓度在25～200μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9996);平均回收率为99.57%,RSD=1.91%(n=6)。结论:本方法灵敏、简便、准确,可用于小儿止咳颗粒的质量控制。
|
| 关 键 词： | 小儿止咳颗粒 高效液相色谱法 黄芩苷 含量测定 |
Content Determination of Baicalin in Xiaoer Zhike Granule by HPLC |
| |
| LIAO Wei-ming,LIU Gui-zhen,ZHOU Guo-bo. Content Determination of Baicalin in Xiaoer Zhike Granule by HPLC[J]. China Pharmacy, 2010, 0(31): 2946-2947 |
| |
| Authors: | LIAO Wei-ming LIU Gui-zhen ZHOU Guo-bo |
| |
| Affiliation: | (Shenzhen Baoan District People’s Hospital, Shenzhen 518000, China) |
| |
| Abstract: | OBJECTIVE： To establish the HPLC method for the content determination of baicalin in Xiaoer zhike granule. METHODS： YWG C18 （150 mm×4.6 mm,10 μm） column was used and the mobile phase consisted of methnol-water-phosphoric acid （47 ∶ 53 ∶ 0.2） with the flow rate of 2.5 mL·min-1. The detection wavelength was set at 280 nm and injection volume was 10 μL. RESULTS： The linear range of baicalin was 25～200 μg·mL-1（r=0.999 6） with an average recovery of 99.57%（RSD=1.9%,n=6）. CONCLUSION： This method is sensitive, simple and accurate for the quality control of Xiaoer zhike granule. |
| |
| Keywords: | Xiaoer zhike granule HPLC Baicalin Content determination |
| 本文献已被 维普 等数据库收录！ |
|
