紫荆花黄酮类化合物的HPLC指纹图谱研究

目的:建立紫荆花黄酮类化合物的高效液相色谱(HPLC)指纹图谱,为其采收、利用及质量评价提供依据。方法:以芦丁、槲皮素2种标准品为对照,采用反相(RP)-HPLC法测定10批不同产地和不同采收期的紫荆花黄酮类化合物,绘制色谱图,确立参照指纹图谱,并用中药指纹图谱相似度评价系统计算其相似度。色谱条件:色谱柱为ODSC1(8250mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸水溶液(梯度洗脱),柱温为25℃,流速为0.7mL·min-1,检测波长为360nm,记录时间为40min。结果:共标示出紫荆花黄酮类化合物的14个特征指纹峰;各批样品相似度介于0.88～0.94之间。表明紫荆花黄酮类化合物组成相似,但含量有差异。结论:所建立的紫荆花黄酮类化合物HPLC指纹图谱稳定、可靠、重复性好,对紫荆花采收、利用及质量评价具有参考价值。

HPLC Fingerprint of Flavonoids from Bauhinia blakeana

OBJECTIVE： To establish HPLC fingerprint of flavonoids from Bauhinia blakeana and to provide reference for harvest, application and quality evaluation of B. blakeana. METHODS： RP-HPLC method was used to determine the content of flavonoids from 10 batches of B. blakeana from different habitats and in different harvest periods with rutin and quercetin as control. The similarity was calculated using TCM fingerprint similarity evaluation system. The separation was performed on Hypersil ODS C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-0.4% phosphoric acid solution （gradient elution） at flow rate of 0.7 mL·min-1 lasting for 40 min. The UV detection wavelength was set at 360 nm and column temperature was at 25℃. RESULTS： The results showed that 14 peaks were common. The similarity index of 10 batches B. blakeana samples were between 0.88 and 0.94. The results indicated chemical components were similar but content of the compounds was different. CONCLUSION： Established HPLC fingerprint of B. blakeana is stable, reliable and reproducible, which is valuable for harvest, application and quality evaluation of B. blakeana.