| HPLC法测定自发性高血压大鼠血浆中去甲肾上腺素的含量 |
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| 引用本文： | 梁秋云,黄慧学,刘华钢,蒙华琳.HPLC法测定自发性高血压大鼠血浆中去甲肾上腺素的含量[J].中国药房,2010(21):1952-1953. |
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| 作者姓名： | 梁秋云 黄慧学 刘华钢 蒙华琳 |
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| 作者单位： | [1]广西医科大学,南宁市530021 [2]广西中医学院,南宁市530021 [3]广西柳州医学高等专科学校,柳州市545006 |
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| 基金项目： | 广西科技创新能力与条件建设资助项目（桂科基0639046） |
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| 摘 要： | 目的:建立高效液相色谱-紫外检测(HPLC-UV)法测定自发性高血压大鼠(SHR)血浆中去甲肾上腺素(NA)的含量。方法:色谱柱为C18,流动相为甲醇-0.15mo·lL-1磷酸二氢钾缓冲液(1∶9,V/V,pH6.0),检测波长为280nm,柱温为室温,流速为1.0mL·min-1。结果:NA检测浓度线性范围为0.5～40μg·L-1(r=0.9999),最低检测浓度为0.05μg·L-1,平均回收率为97.49%,RSD小于3.81%。结论:本方法线性、精密度、回收率良好,灵敏度高,操作简便,可用于NA的药动学及相关研究。
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| 关 键 词： | HPLC-UV法 自发性高血压 大鼠 去甲肾上腺素 含量测定 |
Content Determination of Noradrenaline in Plasma of Spontaneous Hypertension Rats by HPLC |
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| LIANG Qiu-yun,LIU Hua-gangGuangxi Medical University,Nanning,China HUANG Hui-xueGuangxi College of Traditional Chinese Medicine,Nanning,China MENG Hua-lin.Content Determination of Noradrenaline in Plasma of Spontaneous Hypertension Rats by HPLC[J].China Pharmacy,2010(21):1952-1953. |
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| Authors: | LIANG Qiu-yun LIU Hua-gangGuangxi Medical University Nanning China HUANG Hui-xueGuangxi College of Traditional Chinese Medicine Nanning China MENG Hua-lin |
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| Institution: | LIANG Qiu-yun,LIU Hua-gang(Guangxi Medical University,Nanning 530021,China) HUANG Hui-xue(Guangxi College of Traditional Chinese Medicine,Nanning 530021,China) MENG Hua-lin(Liuzhou Medical College of Guangxi,Liuzhou 545006,China) |
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| Abstract: | OBJECTIVE： To establish an HPLC-UV method for the content determination of noradrenaline （NA） in plasma of spontaneous hypertension rats （SHR）.METHODS： The determination was performed on C18 column with mobile phase consisted of methanol-0.15 mol·L-1 potassium dihydrogen phosphate buffer （1 ∶ 9,V/V,pH 6.0） at flow rate of 1.0 mL·min-1.The detection wavelength was set at 280 nm and column temperature was room temperature.RESULTS： The linear range of NA was 0.5～40 μg·L-1 （r=0.999 9） with an average recovery of 97.49% （RSD3.81%）.The detection limit was 0.05 μg·L-1.CONCLUSION： The method is sensitive and simple with good linearity,precision and recovery for pharmacokinetic study and relevant research of NA. |
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| Keywords: | HPLC-UV methods Spontaneous hypertenision Rats Noradrenaline Content determination |
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