液相色谱-质谱联用法检测中药中非法添加的硝苯地平、尼群地平和尼莫地平

目的:建立快速、准确、高灵敏度的检测中药中非法添加硝苯地平、尼群地平和尼莫地平的分析方法。方法:采用液相色谱-质谱联用法。通过相对分子质量、二级质谱碎片信息、液相色谱保留时间和紫外光谱4方面信息,对中药降压制剂的提取液进行液相色谱-质谱分析。通过与标准品的光谱、色谱及质谱行为相比较,对中药降压制剂中非法掺入的合成降压药进行定性鉴别。结果:结合4方面信息,发现在3种受试中药降压制剂中分别掺有硝苯地平、尼群地平和尼莫地平。结论:该方法选择性强,灵敏度高,可用于分析检测中药降压制剂中非法掺入的硝苯地平、尼群地平和尼莫地平。

Detection of Nifedipine,Nitrendipine and Nimodipine Added in Chinese Patent Medicine Illegally by LC-MS

OBJECTIVE： To develop a rapid, accurate and sensitive method for the determination of nifedipine, nitrendipine and nimodipine which were added into traditional patent medicine illegally. METHODS： The LC-MS method was used to detect the extractive of Chinese patent medicine for antihypertension in respects of relative molecular mass, tandem mass spectrometry fragment, retention time, UV spectrum. The compounds added into the Chinese patent medicine were identified by comparing with standard sample in terms of spectrum, chromatographic and mass spectrometric behavior. RESULTS： According to four aspects of determination, nifedipine, nitrendipine and nimodipine were found in three kinds of Chinese patent medicine for antihypertension. CONCLUSION： The method is selective and sensitive for the detection of nifedipine, nitrendipine and nimodipine which were added into traditional patent medicine illegally.