HPLC-MS/MS法同时测定三七须根总皂苷中人参皂苷Rg_1和Re的含量

目的:建立以液-质联用法同时测定三七须根总皂苷中人参皂苷Rg1和Re含量的方法。方法:色谱柱为BDS HYPERSUL C18(150mm×2.1mm,5μm),流动相为乙腈-水(梯度洗脱),柱温为30℃;采用负离子多反应监测方法(MRM)测试。用于定量分析的对照品离子对分别为人参皂苷Rg1:m/z799.6→475.5;人参皂苷Re:m/z945.8→783.7;内标物紫杉醇m/z:852.5→525.3。结果:人参皂苷Rg1、人参皂苷Re标准曲线的线性范围分别为0.173～17.3μg·mL-1和0.156～39.1μg·mL-1,精密度和准确度等均符合样品分析的要求。结论:本方法准确、灵敏、特异性强,适用于三七须根总皂苷及其制剂中人参皂苷Rg1、Re含量的同时测定。

Simultaneous Determination of Ginsenoside Rg_1 and Ginsenoside Re in Total Saponins of Fibrous Roots of Panax notoginseng by HPLC-MS/MS

OBJECTIVE： To establish a HPLC-MS/MS method for simultaneous determination of ginsenosides Rg1 and ginsenosides Re in total saponins of fibrous roots of Panax notoginseng. METHODS： BDS HYPERSUL C18（150 mm×2.1 mm,5 μm） column was used with mobile phase consisted of acetonitrile-water （gradient elution）. The column temperature was set at 30 ℃. The ginsenosides Rg1 and ginsenosides Re were detected by the negative electrospray ionization mode. Ion pair of reference substance in quantitative determination were as follows： Rg1： m/z 799.6→475.5; Re：m/z 945.8→783.7; paclitaxel： m/z 852.5→525.3. RESULTS： The linear range of ginsenosides Rg1 and ginsenosides Re were 0.173～17.3 μg·mL-1 and 0.156～39.1 μg·mL-1 respectively. The precision and accuracy were in accordance with the requirements. CONCLUSION： The method is accurate, sensitive and specific for simultaneous determination of ginsenosides Rg1 and ginsenosides Re in total saponins of fibrous roots of P. notoginseng.