去甲斑蝥素对多发性骨髓瘤细胞周期和血管内皮细胞生长因子的影响

目的 探讨去甲斑蝥素(NCTD)抗骨髓瘤效应的机制.方法 利用流式细胞术检测不同浓度NCTD作用24h后细胞周期的改变,Western blot检测CDK1、cyclin A和cyclin B1的改变,免疫组化和RT-PCR测定血管内皮细胞生长因子(VEGF)的表达.结果 经NCTD处理24h后,随着NCTD浓度增加,G2/M期细胞明显增加,S期细胞明显减少,呈剂量依赖性;NCTD以剂量依赖的方式抑制cyclin B1、CDK1和cyclin A的表达;NCTD以剂量依赖的方式抑制VEGF mRNA和蛋白的表达.结论 NCTD对U266细胞有抗增殖和促凋亡作用,其作用机制可能与调节细胞周期和抑制VEGF的表达有关.

Effect of norcantharidin on vascular endothelial growth factor and cell cycle of general lymphadenomatosis of bones

Objective To explore the anti-myeloma effect of norcantharidin(NCTD) and the possible mechanism.Methods U266 cells were treated with 0,5,10 and 20 μmol/L NCTD for 24 h.Flow cytometry was used to determine cell cycle distribution.The protein expressions of CDK1,cyclin A and cyclin B1 were determined by Western blot.RT-PCR and immunohistochemistry were used to determine the expression of vascular endothelial growth factor(VEGF).Results U266 cells were markedly arrested at G2/M phase after treatment with different concentration of NCTD after 24 hours,while the cells in S phase decreased from(51.53±6.9)% to(17.6±1.3)%;NCTD inhibited the protein expressions of cyclin B1,CDK1 and cyclin A in dose-dependent manner(P<0.05);the protein and mRNA expressions of VEGF were down-regulated in dose-dependent manner(P<0.05).Conclusions NCTD displays potent antiproliferative activity through suppressing cell cycle proteins,arresting cell cycle at G2/M phase and inhibiting the expression of VEGF.