CCR6-CCL20对食管鳞癌患者体内Th17细胞分布的影响

目的研究CCR6-CCL20对食管鳞癌患者体内Th17细胞分布的影响。方法采用流式细胞术检测食管鳞癌患者手术切除的癌组织、癌旁组织及外周血中Th17细胞的比例及CCR6+Th17细胞的比例。RT-PCR方法检测组织中CCL20 mRNA水平。结果与癌旁组织及外周血相比,癌组织中Th17细胞明显升高(P<0.01);癌组织中CCR6+Th17细胞的比例高于癌旁组织及外周血(P<0.01);癌组织中CCL20 mRNA水平高于癌旁组织(P<0.01),并与Th17呈正相关。结论 CCR6-CCL20趋化轴可能在食管鳞癌患者Th17细胞浸润到肿瘤组织中发挥重要作用。

Effects of CCR6-CCL20 on the distribution of Th17 cells in patients with esophageal squamous cell carcinoma

Objective To explore the effects of CCR6-CCL20 on the distribution of Th17 cells in patients with esophageal squamous cell carcinoma.Methods The frequency of IL-17+ T cells within CD4+ cells and CCR6+ cells within Th17 cells were measured by flow cytometry in tumor tissues,tumor-free tissues and peripheral blood.Real-time PCR was used to detected CCL20 mRNA.Results Compared with corresponding tumor-free tissue and peripheral blood,the levels of Th17 cells were significantly increased in tumor tissues(P<0.01).In addition,the frequency of CCR6+ cells among Th17 cells within tumor tissues were higher than those of tumor-free tissues and peripheral blood(P<0.01).Furthermore,the mRNA levels of CCL20 in tumor tissues were significantly higher than those of corresponding tumor-free tissues(P<0.01),moreover,the levels of Th17 cells were positively correlated with CCL20 mRNA levels.Conclusion The CCR6-CCL20 axis may play an important role in the Th17 cells infiltrating into the tumor tissues.