缺血预处理对大鼠局灶性脑损伤后线粒体Ca~(2+)、细胞色素C的影响

目的观察缺血预处理对大鼠局灶性脑损伤后线粒体Ca2+、细胞色素C的影响。方法用大鼠右侧大脑中动脉阻闭制成局灶性脑缺血模型。24只大鼠,每组8只,随机分为缺血预处理组、模型组和假手术组。缺血预处理组给予30 min预缺血,72 h再灌注,后续2 h缺血,4 h再灌注。用张均田的改良方法测定细胞色素C含量。用火焰原子吸收法检测线粒体Ca2+含量。结果缺血预处理组动物的细胞色素C、Ca2+,与假手术组相比差异显著(P0.05,P0.01),缺血预处理组与模型组相比差异显著(P0.05,P0.01)。结论缺血预处理减少线粒体释放细胞色素C,维持线粒体Ca2+稳态

Effect of ischemic preconditioning on contents of cytochrome C and mitochondrial calcium in rats after focal cerebral ischemic-reperfusion

Objective To observe the effect of ischemic preconditioning on contents of cytochrome C and mitochondrial calcium in rats after focal cerebral ischemic-reperfusion.Methods Focal cerebral ischemic model was made by occlusion of right middle artery in Wistar rats（ischemia for 2 h and reperfusion for 4h）.Rats were randomly divided into three groups：preischemia、model and sham operation.Rats in preischemia group were undergone transient ischemic preconditioning（30 min） and reperfusion（72 h）.The contents of cytochrome C were measured according to Zhangjuntian＇s improved means.The contents of mitochondrial calcium were detected by flame atom absorption.Results The contents of mitochondrial cytochrome C and calcium in model group were significantly lower than sham operation（P〈0.05,P〈0.01）,whereas the contents of plasma cytochrome C were markly higher（P〈0.05,P〈0.01）.The change in preischemia group was obviously difference as compared with the model group.Conclusion Ischemic preconditioning reduced the release of mitochondrial cytochrome C and maintained mitochondrial calcium dyshomeostasis.