去势前后大鼠腹叶前列腺形态学的动态改变

目的：探讨去势前后大鼠腹叶前列腺形态学和组织学的变化规律，以及引起这种变化的机制。方法：将90只SD系成年雄性大鼠分为9组，其中一组作为正常对照，其余8组去势后分别于术后第1、2、3、5、7、10、14、21天处死，测血清雄激素浓度（T），腹叶前列腺的重量、体积和密度。再行石蜡包埋切片，HE染色，采用计算机图像分析系统计算腺体面积与间质面积之比值（G）、管腔分泌物与管腔面积这比值（S）、腺细胞高度（H）及单个腺体模截面细胞数（N）。TUNEL检测细胞凋亡，免疫组织化学方法测定增殖抗原PCNA。结果：去势后T急剧下降，前列腺重量和体积进行性减小，而密度先稍降低再逐渐升高。G、S、H、N均减小。腺上皮细胞凋亡、坏死，增殖下降。结论：去势后大鼠腹叶前列腺重量和体积的减小，可能是由于细胞数目减少、细胞萎缩、细胞分泌物减少等综合作用的结果，其始动因素可能是T水平的降低。

Morphological changes of rats' ventral prostates following castration

Purpose:We performed the research to reveal the morphologic changes of rats' ventral prostates pre and post castration, and explored the possible mechanism which caused these morphologic changes thoroughly.Methods: 90 male adult rats were randomized to 9 groups(10 rats per group), one group for the control, while the others were sacrificed in 1, 2, 3, 5, 7, 10, 14, 21 days post castration. The serum testosterone concentrations were detected, and prostates were weighed and volumed, then fixed, embedded, sectioned, stained by H.E, and observed by light microscope. Furthermore, sections of prostates were detected apoptosis by TUNEL and proliferation by immunohistochemistry. Sections stained by H.E. were applied to Computer-based imagine analysis system in order to count the area of glands and stroma as well as the width of the epithelium.Results: Serum testosterone concentrations were decreased rapidly, while prostatic weights and volumes were reduced, and the prostatic glands were atrophied, and the secretion of glands was diminished as time passed. Apoptosis and necrosis occurred in the epithelia as well as in the stromal cells of rats' prostates, and proliferation index was decreased. Conclusions: Weight and Volume loss of the castrated prostates was due to the loss and atrophy of the prostate cells as well as the decrease of secretion. These changes might result from low level of the serum testosterone.