SLE和PSS患者的自身免疫抗体检测及临床价值

目的:探讨自身免疫抗体ANA、ENA多肽抗体及抗ds-DNA对系统性红斑狼疮(SLE)和原发性干燥综合征(PSS)的诊断价值。方法:检测110例SLE和PSS患者血清,ANA采用间接免疫荧光法,抗ENA抗体用免疫印迹法,ds-DNA用免疫胶体金技术检测。结果:SLE患者ANA阳性率最高(90.0%),PSS患者抗SSA阳性率最高(80.3%),两组患者中抗Sel-70阳性率最低,分别为1.9%和1.8%。结论:自身免疫性疾病患者体内常存在有一种或多种高效价的自身抗体,SLE和SS患者各有特异性抗体存在,联合检测多种自身免疫抗体,对临床诊断有重要意义。

The Clinical Significance of Autoimmune Antibodies in Systemic Lupus Erythematosus and Primary Sjogren's Syndrome

Objective To investigation the significance of autoimmune antibodies ANA, anti-ENA antibody, ds-DNA antibody in systemic lupus erythematosus (SLE) and primary Sjogren's syndrome (PSS). Methods To detect 110 serum samples of SLE and PSS patients. ANA was detected by immunofluorescence assay, anti-ENA antibody was detected by immunoblot method, ds-DNA was detected by colloidal gold method. Results The positive rate of ANA was the highest (90.0%) in the SLE patients, the positive rate of anti-SSA was the highest (80.3%) in the PSS patients, while the positive rate of antiScl-70 was lower in the both, they were 1.9% and 1.8% respectively. Conclusion There were more than one autoimmune antibodies in patient's serum of autoimmune diseases. Characteristic antibody exists in the LSE and PSS. Simultaneously detection manifold autoimmune antibody was important to clinical diagnosis.