| 用正交设计法优选老鹰茶总黄酮的提取工艺 |
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| 引用本文： | 程文明,王小华,陆维丽,李俊. 用正交设计法优选老鹰茶总黄酮的提取工艺[J]. 安徽医药, 2005, 9(10): 729-730 |
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| 作者姓名： | 程文明 王小华 陆维丽 李俊 |
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| 作者单位： | 安徽医科大学药学院,安徽,合肥,230032;安徽医科大学药学院,安徽,合肥,230032;安徽医科大学药学院,安徽,合肥,230032;安徽医科大学药学院,安徽,合肥,230032 |
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| 基金项目： | 安徽省自然科学基金 , 安徽省人才基金 |
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| 摘 要： | 目的优选老鹰茶总黄酮提取的最佳工艺.方法以芦丁为对照,AlCl3为显色剂,用紫外分光光度法在410 nm处测定总黄酮含量,以总黄酮含量为指标,应用正交实验,优选老鹰茶总黄酮提取的最佳工艺.结果老鹰茶总黄酮的最佳提取工艺为80%乙醇,12倍量,回流提取3次,每次2 h.结论紫外分光光度法方便、快捷,可用于老鹰茶总黄酮提取工艺的优选.
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| 关 键 词： | 老鹰茶 总黄酮 正交试验 提取工艺 紫外分光光度法 |
| 收稿时间： | 2005-07-25 |
| 修稿时间： | 2005-07-25 |
The optimization of the extracting method of total flavonoids from Litsea coreana through orthogonal test |
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| CHENG Wen-ming,WANG Xiao-hua,LU Wei-li,LI Jun. The optimization of the extracting method of total flavonoids from Litsea coreana through orthogonal test[J]. Anhui Medical and Pharmaceutical Journal, 2005, 9(10): 729-730 |
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| Authors: | CHENG Wen-ming WANG Xiao-hua LU Wei-li LI Jun |
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| Abstract: | Aim To optimize the extraction procedure of the total flavo noids from Litsea coreana. Methods The optimized extraction proc edure was screened by orthogonal test according to the content of the total flav onoids which was measured through UV spectroscopy at 410 nm using rutin as contr ol, AlCl_(3) as reagent. Results The optimized extraction procedu re was as follows: 80% alcohol, 12 times volume of the weight of the dried mater ial, refluxing 2 h for 3 times. Conclusion UV spectroscopy is a fast and convienient method suitable for the control of the extraction of the total flavonoids from Litsea coreana. |
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| Keywords: | Litsea coreana total flavonoids orthogonal test extraction procedure UV spectroscopy |
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