糖槭叶中皂苷的提取分离条件探讨

目的：研究糖槭叶中皂苷的提取分离条件，为糖槭中皂苷的开发提供科学依据。方法：乙醇浸渍提取法，大孔吸附树脂分离法和薄层色谱法（TLC）。结果：在大孔吸附树脂分离过程中，用10％乙醇作流动相可以分离得到糖、皂苷、香豆素；用30％乙醇作流动相可以分离得到糖、皂苷、香豆素；用50％乙醇作流动相可以分离得到糖、皂苷、香豆素、黄酮苷；用70％乙醇作流动相可以分离得到糖、皂苷；用90％乙醇作流动相可以分离得到皂苷。结论：80％乙醇浸渍法提取，大孔吸附树脂分离皂苷部位方法最佳。

Extraction and separation of saponins from the Acer saccharum leaf

Objective:To study extraction and separation of saponins from the leaf of the Acer saccharum and provide the scientific basis for the development of separation of glucoside from Acer saccharum.Method:We used 80% ethyl alcohol immersion method,macroporous resin separation method and the thin layer chromatography(TLC) method.Result:When we separated saponin from the Acer saccharum leaf with macroporous resin,we obtained the amylase,saponin and coumarin by using 10% ethyl alcohol mobile phase,obtained the amylase,saponin and coumarin by using 30% ethyl alcohol mobile phase,obtained the amylase,saponin,coumarin and favonoid glycoside by using 50% ethyl alcohol mobile phase,obtained the amylase and saponin by using 70% ethyl alcohol mobile phase and obtained the aponin by using 90% ethyl alcohol mobile phase.Conclusion:Extracting efficient components from the Acer saccharum leaf by 80% ethyl alcohol immersion method and separating saponin from the Acer saccharum leaf with macroporous resin are the optimal methods.