| 双歧杆菌的完整肽聚糖对LPS激活裸鼠腹腔巨噬细胞的影响 |
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| 引用本文： | 王立生,潘令嘉,施理,孙勇,张亚历,周殿元. 双歧杆菌的完整肽聚糖对LPS激活裸鼠腹腔巨噬细胞的影响[J]. 中华微生物学和免疫学杂志, 2000, 20(1): 4-6 |
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| 作者姓名： | 王立生 潘令嘉 施理 孙勇 张亚历 周殿元 |
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| 作者单位： | 第一军医大学南方医院全军消化内科研究所 |
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| 摘 要： | 目的 了解被ＬＰＳ激活的裸鼠腹腔巨噬细胞在用双歧双歧杆菌的完整聚糖刺激后产生的ＮＯ、ｉＮＯＳ及ｃＧＭＰ的水平。方法 以Ｇｒｉｅｓｓ试剂、激光截聚焦显微镜以及入免法分别测定裸鼠腹腔巨噬细胞产生的ＮＯ、ｉＮＯＳ及ｃＧＭＰ的水平。结果 完整肽聚糖注射组裸鼠腹腔巨噬细胞在ＬＰＳ诱导下产生ＮＯ、ｉＮＯＳ及ｃＧＭＰ含量均显著高于对照组（Ｐ〈０．０１）。结论 双歧双歧杆菌的完整肽聚糖能协同ＬＰＳ激活巨噬细胞，使
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| 关 键 词： | 双歧杆菌 肽聚糖 巨噬细胞 脂多糖 |
| 修稿时间： | 1998-11-30 |
Effects of whole peptidoglycans of Bifidobacteria on signal effectors produced by peritoneal macrophages of nude mice |
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| WANG Lisheng,PAN Lingjia,SHI Li,et al.. Effects of whole peptidoglycans of Bifidobacteria on signal effectors produced by peritoneal macrophages of nude mice[J]. Chinese Journal of Microbiology and Immunology, 2000, 20(1): 4-6 |
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| Authors: | WANG Lisheng PAN Lingjia SHI Li et al. |
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| Affiliation: | WANG Lisheng,PAN Lingjia,SHI Li,et al. PLA Institute for Digestive Medicine,Nanfang Hospital,The First Military Medical University,Guangzhou 510515,P.R.China Corresponding autor:WANG Lisheng,E mail:jinrm @ fimmu.edu.cn |
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| Abstract: | Objective To study the levels of nitric oxide(NO), inducible nitric oxide synthase(iNOS) and cGMP produced by peritoneal macrophages of nude mice after stimulation by whole peptidoglycans of Bifidobacteria bifidum. Methods The content of NO, iNOS and cGMP derived from peritoneal macrophages of nude mice was detected by employing Griess reagent, laser scanning confocal microscopy and radioimmunoassay, respectively. Results The content of NO, iNOS and cGMP derived from peritoneal macrophages of nude mice induced by LPS in whole peptidoglycan injection group was significantly higher when compared with control group(P<0 01). Conclusions Whole peptidoglycans of Bifidobacteria bifidum in synergy with LPS could activate macrophages to produce a large amount of NO, iNOS and cGMP. These signal effectors mediated many kinds of significant physiological functions of whole peptidoglycans. |
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| Keywords: | Bifidobacterium Peptidoglycan Macrophage Lipopolysaccharide |
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