槲皮素,异鼠李素对Cu^2＋介导的LDL氧化修饰的抑制作用

目的：观察槲皮素（Ｑｕｅ）、异鼠李素（Ｉｓｏ）对Ｃｕ＾２＋介导的低密脂蛋白（ＬＤＬ）氧化修饰的影响。方法：采用一次性密度梯度离心法分离正常人血浆脂蛋白，用Ｃｕ＾２＋进行体外氧化修饰，温育前加不同浓度的Ｑｕｅ及Ｉｓｏ。检测脂蛋白中脂质过氧化物（ＬＰＯ）、维生素Ｅ（ＶｉｔＥ）含量及超氧化物歧化酶（ＳＯＤ）活性。结果：在Ｃｕ＾２＋与ＬＤＬ温育前加入Ｑｕｅ、Ｉｓｏ可使ＬＤＬ中ＬＰＯ生成减少，明显延缓 ，Ｖ

THE INHIBITORY EFFECTS OF QUERCETIN AND ISORHAMNETIN ON OXIDATIVE MODIFICATION OF LDL INDUCED BY CU~(2 )

Objective: To observe the effects of quercetin (Que) and Isorhamnetin(Iso) on the oxidative modification of low density lipoprotein(LDL). Methods:Human LDL was prepared by one step ultracentrifuge and then oxidized by Cu 2 in vitro, Que and Iso in different concentrations were added to LDL solutions before incubation with Cu 2 . The contents of lipid peroxide(LPO), Vitamin E and activities of superoxide dismutase(SOD) in LDL were determined. Results: Compared with the ox LDL groups, Que and Iso decreased LPO production but elevated VitE levels and SOD activities in a concentration dependent manner. Conclusions: This findings of the experiment indicat that both Que and Iso Significantly protect LDL against oxidative modification, but Que is better than Iso.