肾舒胶囊对阿霉素肾病大鼠氧自由基及肾小球阴电荷的影响

目的 :探讨肾舒胶囊对阿霉素肾病 (AN)大鼠氧自由基及肾小球阴电荷的影响。方法 :将雄性wistar大鼠 36只随机分至正常对照组 (N组 )、非治疗组 (A组 )、肾舒胶囊治疗组 (S组 )。采用单次尾静脉注射阿霉素建立AN模型 ,研究肾舒胶囊对AN大鼠 2 4h尿蛋白排泄率、血清总蛋白 (TP)、白蛋白 (ALB)、总胆固醇 (TC)、甘油三酯(TG)、血清及肾组织超氧化物歧化酶 (SOD)、丙二醛 (MDA)等的影响 ,并观察肾组织病理改变及胶样铁染色情况。结果 :与A组比 ,S组大鼠 2 4h尿蛋白排泄率第 7d始明显降低 (P <0 .0 5 ,P <0 .0 1) ,4周末血清TP、ALB升高 (P <0 .0 1) ,TC、TG低 (P <0 .0 1) ,血清及肾组织SOD活力升高 (P <0 .0 1) ,MDA降低 (P <0 .0 1)。胶状铁染色及肾组织损伤较A组明显改善。结论 :肾舒胶囊具有抗脂质过氧化 ,改善或修复肾小球阴离子屏障损伤作用 ,从而对AN肾病大鼠具有保护作用

STUDY ON THE EFFECT OF SHENSHU CAPSULES ON OXYGEN FREE RADICAL AND NEGATIVE CHARGE OF GGLOMERUL IN ADRIAMYCIN NEPHROSIS RATS

Objective : To investigate the effect of Shenshu capsule(SSC) on oxygen free radical and negative charge of glomeruli in adrimycin nephrosis(AN) rats . Methods : 36 male Wistar rats were randomly divided into 3 groups : normal control group(N group) , untreated group(A group) , and SSC group(S group) . Adriamycin was injected into the rats through caudal vein to establish AN model . 24 hours' excretion quantity of urine protein(EQUP) was determined , total protein(TP) , albumin(ALB) , total cholesterin(TC) , triglyceride(TG) in serum , superoxide dismutase(SOD) , malondialdehyde(MDA) in serum and renal tissue were detected , and pathological change of renal tissue and the condition of colloidal iron staining were observed . Results : EQUP , TC , TG , MDA in S group were significantly lower than those in A group(P<0.01) , while TP , ALB , SOD were remarkably higher(P<0.01) . Damage of renal tissue in groups S is slighter compared with that in group A . Conclusion : SSC can inhibit lipid peroxidation and, alleviate the injury of glomeruli anion barrier , thus protecting AN rat .