Identification and subcellular localization of a putative cell-to-cell transport protein from red clover mottle virus |
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Authors: | M Shanks K Tomenius D Clapham N S Huskisson P J Barker I G Wilson A J Maule G P Lomonossoff |
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Affiliation: | John Innes Institute, Norwich, United Kingdom. |
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Abstract: | To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic oligopeptide, we have detected a 43-kDa protein in the 30,000 g pellet from extracts of RCMV-infected cowpea protoplasts. Immunogold cytochemistry further localized this protein to the plasmodesmata of RCMV-infected pea tissue. This subcellular location, taken together with other evidence, suggests that this 43-kDa protein has a role in the cell-to-cell spread of RCMV. |
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