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Specific shRNA targeting of FAK influenced collagen metabolism in rat hepatic stellate cells |
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| Authors: | Zhi-Na Dun Xiao-Lan Zhang Jun-Yan An Li-Bo Zheng Robert Barrett and Shu-Rui Xie |
| Institution: | Zhi-Na Dun, Xiao-Lan Zhang, Jun-Yan An, Li-Bo Zheng, Robert Barrett, Shu-Rui Xie, Department of Gastroenterology, The Second Hospital of Hebei Medical University, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology, Shijiazhuang 050000, Hebei Province, China;Robert Barrett, Inflammatory Bowel Disease Center and Immunobiology Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States |
| Abstract: | AIM:To investigate the effects and mechanism of disruption of focal adhesion kinase(FAK) expression on collagen metabolism in rat hepatic stellate cells(HSC).METHODS:The plasmids expressing FAK short hairpin RNA(shRNA) were transfected into HSC-T6 cells,and the level of FAK expression was determined by both real-time quantitative polymerase chain reaction(QPCR) and Western blotting analysis.The production of type collagen and type collagen in FAK-disrupted cells was analyzed by real-time Q-PCR.The level of ... |
| Keywords: | Focal adhesion kinase Hepatic stellate cells Matrix metalloproteinases RNA interference Type I collagen Type III collagen Tissue inhibitors of metalloproteinases |
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