心钠素免疫反应物质在豚鼠耳蜗Corti器中的分布

目的 为了解豚鼠耳蜗Ｃｏｒｔｉ器组织中心钠素免疫反应（Ａｔｉａｌ ｎａｔｒｉｕｒｅｔｉｃ ｐｅｐｔｉｄｅｓ ｉｍｍｕｎｏｒｅａｃｔｉｏｎ,ＡＮＰ－ＩＲ）物质的分布及形态学特征。方法 对耳蜗切片组织采用免疫组织化学ＡＢＣ法进行研究。结果 Ｃｏｒｔｉ器中内侧第一、二排外毛细胞,Ｂｏｅｔｔｃｈｅｒ细胞和靠近螺旋韧带嵴的基底膜ＡＮＰ－ＩＲ为阴性,其余的组织细胞均为阳性反应。结论 ＡＮＰ在Ｃｏｒｔｉ器功能方

Study of Immunohistochemical Distribution and Cell Location of Atrial Natriuretic Peptides in the Corti's Organ of Guinea Pigs

Objective To study the distribution and cell location of the atrial natriuretic peptides immunoreaction (ANP-IR)in the Corti's organ in cochlea of guinea pigs.Methods The immunhistochemical technique (ABC method)was taken on cochlea tissue.Results ANP-IR was found in parts of Corti's organ,but ANP-IR was neg ative in the two medial lines of the outer hair cell,Boettcher's cells and basement membrane near spiral crest .Conclusion This paper suggests that ANP maybe act as a neurotransmiter or modulator in auditory pathway .The function the outer hair cells in each line may be distinction because of different result of ANP-IR among the three lines.