Immunization with a low dose of hemagglutinin-encoding plasmid protects against 2009 H1N1 pandemic influenza virus in mice |
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Authors: | Fang Jing Chen Zhihui Liu Xiaoqing Li Huaifang Wang Jianjun Shen Xiaona Chen Wei Liu Yuan Wang Yue Zhao Ping Qi Zhongtian |
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Affiliation: | a Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China b Department of Gynaecology and Obstetrics, Tongji Hospital, Tongji University, 389 Xincun Road, Shanghai 200065, China c Department of Infectious Diseases, Affiliated Changhai Hospital, Second Military Medical University, Shanghai 200433, China d Center for Disease Control and Prevention of Fujian Province, 76 Jintai Road, Fuzhou, Fujian Province 350001, China e State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, 100 Yingxin Lane, Xuanwu District, Beijing 100052, China |
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Abstract: | A vaccine against the novel pandemic influenza virus (2009 H1N1) is available, but several problems in preparation of vaccines against the new emerging influenza viruses need to be overcome. DNA vaccines represent a novel and powerful alternative to conventional vaccine approaches. To evaluate the ability of a DNA vaccine encoding the hemagglutinin (HA) of 2009 H1N1 to generate humoral responses and protective immunity, BALB/c mice were immunized with various doses of 2009 H1N1 HA-encoding plasmid and anti-HA total IgG, hemagglutination inhibition antibodies and neutralizing antibodies were assayed. The total IgG titers against HA correlated positively with the doses of DNA vaccine, but immunization with either a low dose (10 μg) or a higher dose (25-200 μg) of HA plasmid resulted in similar titers of hemagglutination inhibition and neutralizing antibodies, following a single booster. Further, 10 μg plasmid conferred effective protection against lethal virus challenge. These results suggested that the DNA vaccine encoding the HA of 2009 H1N1 virus is highly effective for inducing neutralizing antibodies and protective immunity. DNA vaccines are a promising new strategy for the rapid development of efficient vaccines to control new emerging pandemic influenza viruses. |
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Keywords: | Influenza virus Hemagglutinin DNA vaccine Neutralizing antibodies Hemagglutination inhibition antibodies Virus challenge |
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