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Analysis of epitopes in the capsid protein of avian hepatitis E virus by using monoclonal antibodies |
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| Authors: | Dong Shiwei Zhao Qin Lu Mingzhe Sun Peiming Qiu Hongkai Zhang Lu Lv Junhua Zhou En-Min |
| Affiliation: | a Department of Preventive Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai An, Shandong 271018, China b China Animal Health and Epidemiology Center, Qing Dao, Shandong 266000, China c Veterinary Immunobiology Research Institute of Northwest A & F University, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, China |
| Abstract: | Avian hepatitis E virus (HEV) is related genetically and antigenically to human and swine HEVs and capsid protein of avian HEV shares approximately 48-49% amino acid sequence identities with those of human and swine HEVs. Six monoclonal antibodies (MAbs) were produced and used to locate different epitopes in the ORF2 region of aa 339-570 of avian HEV Chinese isolate. The results showed that five epitopes were located in the aa 339-414 region and one in the aa 510-515 region. Two epitopes located in aa 339-355 and aa 384-414 regions are the immunodominant epitopes on the surface of the avian HEV particles as demonstrated by immune capture of viral particles and immunohistochemical detection of the ORF2 antigens with two MAbs. |
| Keywords: | Avian HEV Capsid protein Epitope mapping MAbs |
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