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A new method for detection of pandemic influenza virus using High Resolution Melting analysis of the neuraminidase gene |
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| Authors: | Varillas David Bermejo-Martin Jesus F Almansa Raquel Rojo Silvia Nogueira Begoña Eiros Jose María Rico Lucia Iglesias Verónica de Lejarazu Raul Ortiz |
| Affiliation: | a National Influenza Centre, Valladolid, Spain b Infection & Immunity Unit, Microbiology Service, Clinical University Hospital of Valladolid - IECSCYL, Ramón y Cajal 3, 47005 Valladolid, Spain |
| Abstract: | Diagnostic methods based upon exclusive detection of haemagglutinin do not detect sequence variation in other gene segments of the Influenza A virus. A complementary approach is described based upon high-resolution melting curve analysis of the neuraminidase gene, an approach with the potential ability to detect small changes in the neuraminidase sequence without the need for specific probes. |
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