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Inhibitory effects of tricin derivative from Sasa albo-marginata on replication of human cytomegalovirus |
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| Authors: | Akuzawa Kazuhiko Yamada Rie Li Zhuan Li Ying Sadanari Hidetaka Matsubara Keiko Watanabe Kunitomo Koketsu Mamoru Tuchida Yuuzo Murayama Tsugiya |
| Affiliation: | a Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa 920-1181, Japan b Life Science Research Center, Gifu University, Gifu 501-1193, Japan c Faculty of Engineering, Gifu University, Gifu 501-1193, Japan d Hououdou Co. Ltd., Tokyo 142-0063, Japan |
| Abstract: | The anti-human cytomegalovirus (HCMV) activity of tricin (4′,5,7-trihydroxy-3′,5′-dimethoxyflavone), a derivative from Sasa albo-marginata, was studied in the human embryonic fibroblast cell line MRC-5. In a plaque assay, tricin and ganciclovir (GCV) showed concentration-dependent inhibitory properties from 0.05 to 3.6 μM and 0.01 to 1.0 μM, respectively. Tricin had no virucidal effects on cell-free HCMV. Treatment with tricin 1 h before, or 1 h or 3 h after viral infection significantly suppressed HCMV replication. Moreover, tricin inhibited the expression of immediate early (IE) 2 mRNA and DNA polymerase (UL54) mRNA in HCMV-infected cells. Western blot analysis also demonstrated that tricin decreased the expression of IE antigen (especially IE2) and cyclooxygenase 2 (COX-2) expression in HCMV-infected cells. In the presence of tricin, prostaglandin E2 (PGE2) accumulation by HCMV infection was completely inhibited. These results suggest that tricin is a novel compound with potential COX inhibitor-dependent anti-HCMV activity. |
| Keywords: | Anti-cytomegalovirus activity Tricin HCMV IE2 COX-2 Entry |
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