细胞外基质黏附分子表达与成釉细胞瘤生物学特性的关系

 【目的】探讨细胞外基质黏附分子层粘连蛋白（LN）和纤黏连蛋白（FN）与成釉细胞瘤复发和侵袭的关系。【方法】采用免疫组织化学LABC方法检测28例成釉细胞瘤组织中LN和FN的表达。【结果】在28例成釉细胞瘤中，LN和FN在基底膜的阳性表达率分别为46．4％和39．3％，复发性成釉细胞瘤LN和FN在基底膜的阳性表达率明显低于原发性成釉细胞瘤（P〈0．01）。LN和FN的表达形式相似，阳性着色部位均位于基底膜及部分肿瘤细胞的胞浆，在基底膜连续着色的部位，靠近基底膜的肿瘤外周细胞胞浆染色较强，中央内层细胞胞浆染色弱或无，而在基底膜着色缺失的部位，靠近基底膜的肿瘤外周细胞胞浆染色弱或缺失。【结论】LN和FN在基底膜表达缺失与成釉细胞瘤的复发密切相关，可能是成釉细胞瘤局部侵袭的机制之一。

Relationship Between Expression of Laminin and Fibronectin and Biological Behavior of Ameloblastoma

Objective] To investigate the relationship of expression of laminin and fibronectin with invasion and recurrence of ameloblastoma.Methods]Immunohistochemical LABC method was used in detecting the expression of laminin and fibronectin in 28 cases of ameloblastoma.Results] The total positive expression rates of laminin and fibronectin in the basement membrane were 46.4% and 39.3%.The positive expression rates of laminin and fibronectin in basement membrane of secondary ameloblastoma were significantly higher than those of primary ameloblastoma(P< 0.01).The positive expression of laminin and fibronectin was mainly located in the basement membrane and the cytoplasm of some cells.The stained density of the outer layer cells near to continuous basement membrane was higher than that of the inner layer cells,but the stained density of the outer layer cells near to discontinuous basement membrane was predominantly low or deleted.Conclusion] Absent expression of laminin and fibronectin in basement membrane could be associated with recurrent potentiality of ameloblastoma,and may be a reason of invasion in ameloblastoma.