| 四物汤传统汤剂中5-羟甲基糠醛的含量测定 |
| |
| 引用本文： | 刘月新.四物汤传统汤剂中5-羟甲基糠醛的含量测定[J].中国现代药物应用,2013(17):1-2. |
| |
| 作者姓名： | 刘月新 |
| |
| 作者单位： | 湖南中医药大学药学院／药学基础教学实验中心,410208 |
| |
| 基金项目： | 湖南中医药大学青年教师科研基金课题（项目编号：99820001） |
| |
| 摘 要： | 目的建立紫外分光光度法测定四物汤传统汤剂中5-羟甲基糠醛含量的方法。方法通过测定吸光度,建立标准曲线法,建立浓度和吸光度值关系方程式,计算得不同煎煮时间时5-羟甲基糠醛的浓度。结果 5-羟甲基糠醛在5.262~26.310μg/ml呈良好的线性关系,γ=0.9998(n=5)。测得5-羟甲基糠醛浓度分别为:23.6480μg/ml、25.9654μg/ml、24.6716μg/ml。结论 5-羟甲基糠醛的含量在一定煎煮时间范围内随时间的延长而增加,在80 min时达到最大值。
|
| 关 键 词： | 四物汤 5-羟甲基糠醛 紫外分光光度法 |
The determination of 5-hydroxymethyfurfural in siwu decoctions |
| |
| LIU Yue-xin.The determination of 5-hydroxymethyfurfural in siwu decoctions[J].Chinese Journal of Modern Drug Application,2013(17):1-2. |
| |
| Authors: | LIU Yue-xin |
| |
| Institution: | LIU Yue-xin. (Pharmacy Basic Teaching Experiment Center, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China) |
| |
| Abstract: | Objective To establish a UV spectrophotometry method for the determination of 5-hydroxy- methyl furfural （5-hmf） in the siwu decoctions. Methods The aniline ＇ s standard curve was determined through different absorbance with different concentration. The authors also acquired the relative equation be- tween absorbance and concentration. The concentrations of 5-HMF at decoction different times were calculated. Results the calibration curve was linear in the rang of （ 5. 262 - 26. 310 ） μg/ml, γ = 0. 9998 （ n = 5 ）. The tested 5-HMF concentrations ranged from 23. 6480 μg/ml,25. 9654 μg/ml,24. 6716 μg/ml, respectively. Con- clusion 5-HMF content increased along with the decoction times extension. The concentrations of 5-HMF a- chieved the maximal at reaction time of 80 min. |
| |
| Keywords: | Siwu decoctions 5-hydroxymethyfurfural UV spectrophotometry |
| 本文献已被 CNKI 维普 等数据库收录！ |
