攀枝花地区0～5岁儿童轮状病毒腹泻的分子流行病学研究

目的探讨攀枝花地区2009年10～12月婴幼儿轮状病毒腹泻的分子流行病学特征及临床特点。方法采用胶体金免疫层析法、逆转录-聚合酶链法(RT-PCR)对收集的婴幼儿腹泻标本270份进行轮状病毒检测及A组轮状病毒常见的G、P基因分型研究。结果 270份腹泻标本轮状病毒阳性率为38.6%;6月～2岁年龄组的阳性率为45.4%(89/196),2～5岁年龄组的阳性率为20.3%(15/74),两组对比差异有统计学意义(P﹤0.005);104例被检测的A组轮状病毒腹泻粪便标本中G分型以G2为主,P分型以P4为主。结论轮状病毒是攀枝花地区婴幼儿腹泻的主要病原,G2型和P4型为2009年的优势毒株。该病毒引起的小儿腹泻主要发生在秋冬季,6月～2岁婴幼儿是轮状病毒的易感人群。

Molecular epidmiology of Rotavirus diarrhea among 0-5 young children in Panzhihua

OBJECTIVE To discuss molecular epidemiology and clinic characteristics of rotavirus diarrhea among infants and young children in Panzhihua from October to December in 2009.METHODS Rotavirus antigen of 270 stool specimens collected from infants and young children with diarrhea was detected by using immune colloidal gold method.RT-PCR was used to indentify G and P genotypes of group A rotavirus.RESULTS Rotavirus was detected in 104 of 270 specimens and the rotavirus positive rate was 38.6%.The rotavirus positive rate was 45.4%(89/196)in the group at the age of 6 months to 2 years.The rotavirus positive rate was 20.3%(15/74)in the group at the age of 2 years to 5 years.The difference between them was statistical significant(P﹤0.05).G2 and P4 were the predominant genotypes of rotavirus diarrhea in 104 stool specimens from group A.CONCLUSION Rotavirus was the main pathogen of infants with diarrhea in Panzhihua.G2 and P4 were the prevalent types in 2009.Infants with diarrhea caused by rotavirus mainly occurred in autumn and winter.The children at the age of 6 months to 2 years were the infected group by rotavirus.