九节龙皂苷Ⅰ对小鼠Lewis肺癌和裸鼠肝癌SMMC-7721的抑制作用

目的:研究九节龙皂苷Ⅰ对小鼠Lewis肺癌和裸鼠肝癌SMMC-7721的抑制作用.方法:C57BL/6小鼠足跖接种稀释3倍的Lewis肺癌细胞悬液20μl制备肺转移模型,灌胃给药14 d后,截下荷瘤足,计算原位抑癌率,继续给药至25 d,计算肺转移集落数和转移抑制率;BALB/c/nu裸鼠背部皮下接种肝癌SMMC-7721细胞5×106个/ml复制人肝癌实体瘤模型,连续给药16 d,动态测量肿瘤体积、体重,画肿瘤生长曲线,实验结束后,剥离瘤块,计算抑瘤率.结果:九节龙皂苷Ⅰ 25 mg/kg～100 mg/kg对小鼠Lewis肺癌原位癌抑制率和肺转移均分别在40.5%～54.0%和45.4%～69.1%之间,对裸鼠肝癌SMMC-7721实体瘤的抑制率在45.6%～56.3%之间.结论:九节龙皂苷Ⅰ对Lewis肺癌转移瘤和裸鼠肝癌SMMC-7721均有抑制作用.

Inhibitory Effect of Ardipusilloside-Ⅰ on Lewis Pulmonary Carcinoma and Hepatocarcinoma SMMC-7721

OBJECTIVE: To study the antitumor effect of ardipusilloside-I (ADS-I) on Lewis pulmonary carcinoma and hepatocarcinoma SMMC-7721. METHODS: Lewis pulmonary metastasizing model was made by Sc. diluted 3-time 20 microl cancer cells in the right sole of C57BL/6 mice, after we treated the mice 14 days with ADS-I orally, cut the neoplasia-foot and weighed, we calculated the inhibitory rate in situ, then put the mice to death after being administrated 11 days continuously, counted the lung medtastasis colony and calculated the metastasis inhibitory rate; Human being hepatocarcinoma model was made by sc. in the back 5 x 10(6)/ml SMMC-7721 cells per BALB/c/nu nude mice, after we treated them orally 16 days with ADS-I continuously, measured the volume of tumor growth and body weight, and drew the growth curve, we detached the tumor and calculated the inhibitory rate of tumor growth in the end. RESULTS: Lewis pulmonary carcinoma inhibitory rate in situ and lung transfer inhibitory rate of ADS-I (25 mg/kg - 100 mg/kg) were between 40.5% and 54.0%, 45.4% and 69.1% respectively; Inhibitory rate of hepatocarcinoma SMMC-7721 was between 45.6% and 56.3%. CONCLUSIONS: ADS-I has inhibitory effect on Lewis pulmonary metastasis carcinoma and hepatocarcinoma SMMC-7721 carcinoma.