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L-精氨酸对大鼠血管损伤后内膜增生及相关细胞周期调控基因表达的影响
引用本文:聂晓敏,刘瑞云,黄振文,董建增,张金盈,李庚山. L-精氨酸对大鼠血管损伤后内膜增生及相关细胞周期调控基因表达的影响[J]. 中国病理生理杂志, 2001, 17(3): 248-250
作者姓名:聂晓敏  刘瑞云  黄振文  董建增  张金盈  李庚山
作者单位:1. 南医科大学第一附属医院心内科, 河南 郑州 450052;
2. 湖北医科大学附属第一医院心内科,湖北 武汉 430060
基金项目:河南省重大科技攻关项目(No.971200104)
摘    要:目的:研究L-精氨酸(L-Arg)对大鼠血管损伤后内膜增生及相关细胞周期调控基因表达的影响。方法:大鼠分为假手术组,球囊损伤组(又分为术后48h、7d和14d亚组)及球囊损伤+L-Arg组。取各组实验动脉段测新生内膜面积,并采用免疫组化及计算机图像分析法测细胞周期依赖性激酶2(CDK2)、细胞周期蛋白E(cyclinE)和增殖细胞核抗原(PCNA)的表达水平。结果:球囊损伤后14d组的血浆NO水平低于假手术组(P<0.01),CDK2、cyclinE及PCNA均于球囊损伤术后48h在中膜表达,第7d、14d在内膜表达,中膜几无表达,随着内膜增厚表达水平上升。与球囊损伤后14d组相比,球囊损伤+L-Arg组的血浆NO水平增高(P<0.01),新生内膜面积少59.1%(P<0.01),CDK2、cyclinE及PCNA的阳性表达指数分别低36.1%,46.3%和76.2%(P均<0.01)。结论:L-Arg可有效抑制血管损伤后内膜增生,其机制可能与逆转CDK2、cyclinE及PCNA的过表达从而抑制血管平滑肌细胞增殖有关。

关 键 词:精氨酸  血管成形术  气囊  细胞周期  
文章编号:1000-4718(2001)03-0248-03
收稿时间:1999-12-08
修稿时间:1999-12-08

Effect of L-arginine onintimal proliferation and expression of related cell cycle regulatory factors aftervascular injury in rats
NIE Xiao-min,LIU Rui-run,HUANG Zhen-wen,DONG Jian-zeng,ZHANG Jin-ying,LI Geng-shan. Effect of L-arginine onintimal proliferation and expression of related cell cycle regulatory factors aftervascular injury in rats[J]. Chinese Journal of Pathophysiology, 2001, 17(3): 248-250
Authors:NIE Xiao-min  LIU Rui-run  HUANG Zhen-wen  DONG Jian-zeng  ZHANG Jin-ying  LI Geng-shan
Affiliation:1. Department of Cardiology, the First Affililated Hospital,Henan Medical University,Zhengzhou 450052,China;
2. Department of Cardiology, the First Affiliated Hospital, Hubei Medical University, Wuhan 430060, China
Abstract:AIM:To investigate the effect of L-arginine (L-Arg) on intimal proliferation and expression of related cell cycle regulatory factors after vascular injury in rats. METHODS:Rats were divided into three groups :sham operation group, balloon injury group(this group included balloon 48 h,7 d and 14 d subgroup) and balloon+L-Arg group. Neointima area were calculated morphologiocally. The expression of cyclin dependent kinase-2(CDK2),cyclin E and proliferation cell nuclear antigen (PCNA) were measured by means of immunohistochemical technique and computer image analyzer. RESULTS:After vascular balloon injury, the level of plasma NO decreased, CDK2、cyclin E and PCNA expressed in the media at 48 h and in the neointima at 7 d and 14 d but with low and undetected expression in the media, the expression of CDK2, cyclin E and PCNA increased with the intima thickening. Compared with balloon 14 d group, the plasma NO level increased (P<0.01), the neointima area reduced by 59.1%(P<0.01) and the positive expression indexes of CDK2, cyclin E and PCNA decreased by 36.1%, 46.3% and 76.2% respectively in balloon+L-Arg group (P all<0.01). CONCLUSION:L-Arg can effectively repress intima proliferation after vascular injury, which may be associated with its inhibiting the proliferation of vascular smooth muscle cell through downregulating the excessive expression of CDK2, cyclin E and PCNA.
Keywords:Arginine  Angioplasty   balloon  Cell cycle
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