杜氏利什曼原虫前鞭毛体排泄因子抗原类型的初步研究

利用丙酮提取的排泄因子抗原与前鞭毛体免疫家兔血清以双向免疫扩散法对一株从新疆喀什地区黑热病人分离的杜氏利什曼原虫,一株自陕北病犬中分离的杜氏利什曼原虫,一株由新疆荒漠地区硕大白蛉吴氏亚种消化道中分离的杜氏利什曼原虫和一株沙鼠利什曼原虫进行抗原分析的结果表明,这四株利什曼原虫的排泄因子抗原具有两种不同的抗原成分——P和G。其中喀什绿洲地区黑热病与陕北犬株利什曼原虫均具有P成分,荒漠型黑热病杜氏利什曼原虫具有P和G两种成分。本文讨论了这一初步分型结果的生物学和流行病学意义。

A PRELIMINARY STUDY ON SEROTYPING OF EXCRETED FACTOR OF LEISHMANIA DONOVANII ISOLATED IN XINJIANG, CHINA

Using the Ochterlony double diffusion technique, a preliminary antigen typing was carried out with four leishmanial strains: the L. donovani strain 78-1 isolated from a patient with Kala-azar in Kashkar, Xinjiang, the dog strain of L. donovani isolated from a dog suffered from Leishmaniasis in north Shanxi, the strain 77-1 of L. donovani isolated from the digestive tract of asandfly phlebotomus major wui in a desert type Kala-azar endemic area of Xinjiang, and the strain of L. gerbillae isolated from a gerbil Rhombomis opimus in adesert of Dzungaria Basin.The antigens were prepared by acetone extraction method and the antibodies were raised in rabbits immunized with promastigotes of each leishmanial strain. The results indicated that there were two antigen fractions named fraction P (Pathogenic) and G (Gerbille) in the excreted factor of these leishmanial strains. The strain 78-1 and dog strain of L.donovani shared fraction P, the strain of L. gerbillae had fraction G only, while the strain 77-1, the causitive agent of desert type Kala-azar had both fractions P and G.The biologic and epidemiologic significance of this serotyping is disscused.