浙江省衢州市应用聚合酶链反应检测钩端螺旋体效果评价

目的 建立聚合酶链反应(PCR)检测钩端螺旋体的快速方法,应用于宿主动物钩端螺旋体的快速检测,同时比较PCR方法和常规检测的效果.方法 根据中国疾病预防控制中心提供的引物,以致病性大肠杆菌、产毒性大肠杆菌、溶藻弧菌、沙门菌、霍乱弧菌、痢疾志贺菌、非O1、O139霍乱弧菌、副溶血性弧菌、肠出血性大肠杆菌O157:H7为对照,建立PCR检测钩端螺旋体的快速方法,用于钩端螺旋体的快速检测.结果 衢州市2006年分离钩体菌株进行PCR鉴定结果与血清学鉴定结果相符.100只鼠和100只蛙肝、脾、肾标本进行PCR检测,PCR检测阳性率10%,钩体菌株培养分离率仅为1%,二者差异有统计学意义(2=16.05,P0.005).结论 PCR检测钩端螺旋体灵敏度高、特异性强,可用于钩端螺旋体的快速检测.

Development and application of PCR detection for Leptospire in Quzhou

Objective This study was aimed at developing rapid detection by PCR for leptospire in order to apply this rapid detection on host animals and comparing the effectiveness of the detection by PCR and by convention method. Methods A rapid detection for leptospire by PCR was established with the primers from Chinese Center for Disease Control and Prevention. Enteropathogenic E.coli, Enterotoxigenic E.coli, Vibrio alginolyticus, Saimonella, Cholera bacillus, Shigella dysenteriae, Cholera bacillus of non-O1, O139, Vibrio parahaemolyticus and Enterohemorrhagic E. coli O157:H7 were used as control to detect leptospire rapidly. Results The PCR results of detection for leptospiral strains isolated in Quzhou of 2006 were consisitent with the serological identification. Specimens of liver, spleen and kidney from 100 mice and 100 frogs were detected by PCR, with the positive rate being 10% while the isolation rate was only 1%(2=16.05, P0.005). There was statistical difference between the two groups of data. Conclusion The high sensibility and powerful specificity of the PCR detection made it suitably applied in the detection of leptospire.