阿是止泻胶囊对甲状腺功能亢进性腹泻大鼠杯状细胞的影响

目的 探讨阿是止泻胶囊对甲状腺功能亢进性腹泻(简称甲亢性腹泻)大鼠胃肠道杯状细胞的影响.方法 8周龄的雄性SD大鼠120只,按体质量随机分为对照组(10只)和甲亢性腹泻组(110只).对照组按每只大鼠1ml/d生理盐水灌胃;其余大鼠给予40g/L的甲状腺片混悬液,每只大鼠1 ml/d.根据大鼠血清FT3、FT4水平、体质量和湿粪的数量筛选出40只甲亢性腹泻大鼠.将甲亢性腹泻大鼠按体质量随机分为腹泻对照组、黄连素组,阿是止泻胶囊低剂量组、中剂量组、高剂量组,每组8只.腹泻对照组:按1 ml/d生理盐水给药;黄连素组:按1.94 g·kg-1·d-1给黄连素片;低、中、高剂量组:分别按0.63、1.26、2.52 g·kg-1·d-1给予阿是止泻胶囊.各组连续灌胃7 d,次日脱颈处死,取十二指肠、空肠、回肠、结肠组织,HE染色,光镜观察组织形态及杯状细胞的计数.细胞计数单位为细胞数/高倍镜下每视野(cells/hpf).结果 在空肠中,除低剂量组(23.98±2.28)cells/hpf]外,黄连素组、中剂量组、高剂量组杯状细胞数量(15.32±2.53)、(20.24±1.24)、(14.98±1.10)cells/hpf]均低于腹泻对照组(25.73±4.55)cells/hpf,P均<0.05];结肠中,黄连素组、低剂量组、中剂量组、高剂量组结肠杯状细胞数量(18.29 ±1.33)、(20.61±2.12)、(19.38±2.01)、(16.34±1.55)cells/hpf]均低于腹泻对照组(23.36±3.10)cells/hpf,P均<0.05].空肠和结肠腹泻对照组和高剂量组均低于低剂量组(P均<0.05).十二指肠和回肠中杯状细胞数量组间比较差异无统计学意义(F=2.81、2.67,P均>0.05).形态学观察显示,空场和结肠组织中,腹泻对照组杯状细胞数量明显增多,而随着治疗剂量的增加细胞数量逐渐减少.结论 甲亢性腹泻大鼠空肠和结肠组织中杯状细胞数量明显增多,阿是止泻胶囊能显著降低空肠和结肠组织中杯状细胞数量,减少黏液分泌.

Effect of Asi-antidiarrheal capsule on gastrointestinal goblet cell of thyroid hormone-induced diarrheic rats

Objective To study the effect of Asi-antidiarrheal capsule on gastrointestinal goblet cell of thyroid hormone-induced diarrhea.Methods Total of 120 SD male rats aged about 8 weeks were randomly divided into 2 groups:control group(10 rats)and thyroid hormone-induced diarrheic group(110 rats).Rats in control group were lavaged with normal saline 1 ml/d.Thyroid tablets were partly desolved into normal saline forming a 40 mg/ml suspension.Rats in thyroid hormone-induced diarrheic group were given the thyroid suspension 1 ml/d to make thyroid hormone-induced diarrheic model.Serum FT3 and FT4 were tested.Fourty thyroid hormone-induced diarrheic rats were screened out according to serum FT3 and FT4 levels,body weight and wet stool.The fourty rats were randomly divided into 5 groups,8 rats in each group:positive control group,berberine group,low-dose,mediandose and high-dose groups.Normal saline of 1 ml/d was admnistered to diarrhea control group,1.94 g·kg-1·d-1 Berberine capsule was given to positive control group,and 0.63,1.26,2.52 g·kg-1·d-1 Asi-antidiarrheal capsule to low-dose,mediandose and high-dose groups,respectively.After sever days treatment,rats are executed.Duodenum,jejunum,ileum and colon were dissected,respectively.Histology observation and cell counting were carried out under light micmscopo on HE coloration.Cell counting unit was defined as:cell/high power field of vision (cells/hpf).Results In jejunum,the number of goblet cells in berberine group,mediandose group and high-dose group(15.32±2.53),(20.24±1.24),(14.98±1.10)cells/hpf,respectively],were all lower than that of the diarrhea control group(25.73±4.55)cells/hpf,all P<0.05]with an exception of low-dose group(23.98±2.28)cells/hpf].The numbers of goblet cells in berberine control group,low-dose group,mediandose group and highdose group(18.29±1.33),(20.61±2.12),(19.38±2.01),(16.34±1.55)cells/hpf,respectively]were all less than that of the control group(23.36±3.10)cells/hpf,all P<0.05].The numbers of goblet cells of diarrhea control group and high-dose group were obviously lower than that of the low-dose group(all P<0.05)in jejunum and colon.The numbers of goblet cells of Duodenum and ileum were not significantly different between groups(F=2.81,2.67,all P>0.05).The numbers of goblet cells in the diarrhea control group increased markedly observed under microscope,but decreased following therapeutic treatment.Conclusions The numbers of goblet cells from jejunum and colon in thyroid hormone-induced diarrheic rats are increased significantly.Asi-antidiarrheal capsule can remarkably decrease the number of goblet cells in jejunum and colon,and reduce mucus secretion.