酶联免疫吸附试验检测梅毒抗体的应用评价

目的评价酶联免疫吸附试验（ELISA）在临床应用于梅毒患者诊断的效果。方法采用ELISA方法对住院患者血清进行梅毒抗体检测,收集阳性标本,同时随机收集部分ELISA法检测为阴性样本,使用TPPA试验作为确认试验,从而判断ELISA方法与TPPA检测方法的差异性。结果两种检测方法的一致率较高。ELISA的灵敏度为100%,假阴性率是0,特异性为89.83%,假阳性率是10.17%。假阳性结果主要出现在1≤S/CO〈3的区间内。结论 TP-ELISA能有效应用于梅毒的临床筛查,对于检测结果在1≤S/CO〈3时,应进行确认试验,以减少假阳性。

Application evaluation of ELISA method for detecting specific antibodies of Treponema pallidum

Objective To evaluate the effects of the ELISA method in the diagnosis of Treponemal pallidum(TP) infection.Methods The serum samples from inpatients were detected the TP antibodies by ELISA for collecting the positive samples.At the same time,partial negative samples in ELISA detection were collected randomly.The collected samples were confirmed by the TPPA test.Then the difference between ELISA test and TPPA test was judged.Results The concordance rate of the two detection methods was higher.The sensitivity of ELISA was 100%,the false negative rate was 0,the specificity was 89.83% and the false positive rate was 10.17%.The false positive results occurred mainly in the interval range of 1≤S /Co<3.Conclusion TP-ELISA can be effectively applied in clinical screening of syphilis.If the detection results was in 1 ≤ S/Co <3,the confirmatory test should be conducted for reducing false positive.