不同禁欲时间对体外受精-胚胎移植结局的影响

目的探讨不同禁欲时间对体外受精-胚胎移植(IVF-ET)结局的影响,为辅助生殖技术提供基础数据。方法 535例男性的精液根据取卵日的禁欲时间随机分成A、B、C、D 4组。记录和分析使用各组别的精子进行IVF-ET后的结局。结果在女方年龄、不孕年限、超排卵长方案使用促性腺流毒(Gn)时间和总量以及获卵数没有统计学意义的情况下,2年收集到的A、B、C、D 4组的例数分别为29、159、278、69个。经统计学分析,4组的受精率和卵裂率两两比较差异无统计学意义;C组优质胚胎率显著高于A、B、D组,差异有统计学意义(P<0.05)。种植率各组间二者比较除B组显著高于C组外其余各组间差异无统计学意义;临床妊娠率各组间两两比较,差异均有统计学意义(P<0.05)。临床妊娠中流产率各组间两两比较,差异无统计学意义(P>0.05);异位妊娠率各组间两两比较C组显著高于A、B、D组,差异有统计学意义(P<0.05)外其余各组间没有统计学意义。结论禁欲时间长短不影响体外受精后的受精率和卵裂率,禁欲6～7d的男性精液经密度梯度离心处理用于IVF-ET后其种植率和临床妊娠率较其他禁欲时间低,其原因有待进一步探讨。

The influence of different abstinence time on the outcome of IVF-ET

Objective To investigate the influence of different abstinence time on the outcome of in vitro fertilization-embryo transfer（IVF-ET）,and provide basic data for assisted reproductive technology.Methods Semen of 535 cases were randomly divided into four groups according to abstinence days of the oocyte retrieval day.The outcome of IVF-ET used the sperm from the four different group was recorded and analyzed.Results The cases of Group A（≤3 d）,B（4-5 d）,C（6-7 d）,D（〉7 d） were 29,159,278 and 69.The woman′s age,infertility period,the Gn days and the total number of Gn of super-ovulation,the oocytes numbers in each group were not statistically significant in our research.Data statistical analysis showed that there was no significant difference of the fertilization rate,cleavage rate and the abortion rate among the four groups,The ectopic pregnancy rate and the high-quality embryo rate of group C was significantly higher than that of the group A,B and D（P〈0.05）.There was no statistical significance of the implantation rate between other groups except group B,which was significantly higher than group C,and the results of the clinical pregnancy rate were statistically significant between any two in each group（P〈0.05）.Conclusion The abstinence time（days） did not affect the fertilization rate and cleavage rate after IVF,and the implantation rate and clinical pregnancy rate used the sperm from the abstinence time of 6-7 d for IVF-ET are lower than those in other groups.The reason needs to be further explored.