2型糖尿病及糖尿病肾病患者尿脂质运载蛋白-2检测的意义

目的 探讨2型糖尿病(T2DM)及其合并肾病(DN)尿脂质运载蛋白-2(LCN2)浓度变化及临床意义.方法 根据尿清蛋白排泄率(UAER)将115例T2DM患者分为单纯糖尿病(SDM)组46例、早期糖尿病肾病(EDN)组40例和临床糖尿病肾病(CDN)组29例;另选30例健康体检者作为健康对照(NC)组.采用酶联免疫吸附试验双抗体夹心法检测尿LCN2.结果 SDM、EDN、CDN组尿LCN2、超敏C-反应蛋白(hs-CRP)较NC组升高(P<0.05),随病情加重,升高更为明显.尿LCN2与hs-CRP高度相关(r=0.86,P<0.01).T2DM患者尿LCN2水平随着尿清蛋白的增加而升高.结论 T2DM患者尿LCN2水平明显升高,可作为DN早期的敏感的诊断指标.

Significance of detecting urine lipocalin-2 level in patients with type 2 diabetes mellitus and diabetic nephropathy

Objective To investigate the concentration change and clinical significance of lipocalin-2（LCN2） level in patients with type 2 diabetes mellitus（T2DM） and diabetic nephropathy（DN）.Methods According to urinary albumin excretion rate（UAER）,115 TD2M cases were divided into simple diabetic mellitus（SDM,46 cases）,early diabetic nephropathy（EDN,40 cases） and clinical diabetic nephropathy（CDN,29 cases）;Another 30 healthy persons were taken as the controls（group NC）.Urine level of LCN2 was examined by ELISA with double antibody sandwich method.Results Compared with group NC,urine levels of LCN2 and high sensitive C-reactive protein（hs-CRP） in groups of SDM,EDN and CDN were significantly increased（P〈0.05）,which was more obvious as pathogenetic condition aggravated.ULCN2 was positively correlated with hs-CRP（r=0.86,P〈0.01）.ULCN2 level increased as microalbuminuria increased in T2DM patients.Conclusion The level of urine LCN2 is increased in patients with T2DM,which may be taken as an early sensitive diagnostic marker for DN.