762例妇女宫颈高危型HPV感染与宫颈病变的相关性研究

目的研究妇女宫颈高危型人乳头瘤病毒（hrHPV）感染现状及宫颈病变的感染率,并分析762例妇女年龄与HPV感染及宫颈病变的相关性。方法对762例18～65岁妇女行第2代基因杂交捕获技术（HC2-HPV-DNA）和超薄液基细胞学检测技术（TCT）两种方法联合检测及电子阴道镜下病理活检,并以病理结果为诊断宫颈病变的金标准。结果 762例妇女hrHPV检测阳性率为35.96%（274/762）;病理组织学诊断出宫颈癌及癌前病变（CIN）85例;HPV阳性患者的宫颈癌及癌前病变为79例,敏感性为92.94%（79/85）。hrHPV感染情况在不同年龄段分布差异有统计学意义,hrHPV病毒载量及阳性表达率越高宫颈病变的程度越高。结论 HC2-HPV-DNA检测是目前宫颈癌及癌前病变筛查的最好方法;不同年龄段hrHPV感染情况不同;HC2-HPV-DNA检测hrHPV病毒载量及阳性表达率与CIN以及宫颈癌发生有关,但与宫颈病变的严重程度无关。

Study on correlation between cervical high-risk HPV infection with cervical lesions in 762 women

Objective To study the current status of female cervical high risk human papilloma virus(hrHPV) infection and the infection rate of cervical lesions,and to analyze the correlation between HPV infection with cervical lesions.Methods 762 women aged 18-62 years were performed the combination detection of hybrid capture II(hc2 HPV DNA) and the thinprep cytology test(TCT),and cervical biopsy under electronic colposcopy.The pathological results were as the gold standard for the diagnosis of cervical lesions.Results The overall positive rate of hrHPV detection in 762 women was 35.96%(274/762).Cervical cancer and precancerous lesion cervical intraepithelial neoplasia(CIN) were histopathologically diagnosed in 85 cases.Among the HPV-positive patients,cervical cancer and precancerous lesion were in 79 cases with the sensitivity of 92.94%(79/85).The distribution difference of hrHPV infection among different age groups had statistical significance.The higher the hrHPV viral load and the positive expression rate,the higher the cervical lesion degree.Conclusion HC2-HPV-DNA testing is the best method in screening cervical cancer and precancerous lesions at present.hrHPV infection in different age groups is different.with different distribution.hr-HPV load and the positive expression rate detected by HC2-HPV-DNA are related with the occurrence of CIN and cervical cancer,but not related with the severity of cervical lesions.