| 新基因AngRem104与糖皮质激素受体特异延伸因子的相互作用 |
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| 引用本文： | 张艳玲,张宏,侯平,梁秀彬,崔太根,王海燕.新基因AngRem104与糖皮质激素受体特异延伸因子的相互作用[J].中华肾脏病杂志,2004,20(4):246-249. |
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| 作者姓名： | 张艳玲 张宏 侯平 梁秀彬 崔太根 王海燕 |
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| 作者单位： | 100034,北京大学第一医院肾内科,北京大学肾脏疾病研究所 |
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| 基金项目： | 国家自然科学基金(30370651)，北京大学基因中心科研基金(A17) |
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| 摘 要： | 目的应用免疫共沉淀技术,验证新基因AngRem104和糖皮质激素受体特异延伸因子(GR-EF)蛋白在哺乳动物细胞中的相互作用,为进一步研究AngRem104的生理功能奠定基础。方法构建AngRem104-pcDNA3.1/V5-His和GR-EF-pCMV/c-myc融合表达载体,共转染HEK293T细胞。分别用小鼠抗人c-myc单克隆抗体和小鼠抗人V5-HRP抗体进行免疫沉淀和免疫印迹,以验证AngRem104和GR-EF蛋白间的相互作用。结果AngRem104-V5融合蛋白能够在c-myc抗体的免疫沉淀物中检测出来;在V5抗体的免疫沉淀物中也能检测到GR-EF-c-myc融合蛋白。结论新基因AngRem104和GR-EF蛋白在哺乳动物细胞中存在直接相互作用。
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| 关 键 词： | 糖皮质激素受体 延伸因子 免疫共沉淀 AngRem104 |
| 修稿时间： | 2003年12月25 |
Coimmunoprecipitation analysis of putative interaction of novel gene AngRem104 and glucocorticoid receptor AF-1 specific elongation factor |
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| ZHANG Yan-ling,ZH ANG Hong,HOU Ping,LIANG Xiu-bin,CUI Tai-gen,WANG Hai-yan.Coimmunoprecipitation analysis of putative interaction of novel gene AngRem104 and glucocorticoid receptor AF-1 specific elongation factor[J].Chinese Journal of Nephrology,2004,20(4):246-249. |
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| Authors: | ZHANG Yan-ling ZH ANG Hong HOU Ping LIANG Xiu-bin CUI Tai-gen WANG Hai-yan |
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| Institution: | ZHANG Yan-ling,ZH ANG Hong,HOU Ping,LIANG Xiu-bin,CUI Tai-gen,WANG Hai-yan.Division of Nephrol ogy,Institute of Nephrology,The First Hospital,Peking University,Beijing 1000 34,China Corresponding author:ZHANG Hong,E-mail:hongzh@mail.bjmu.edu.cn |
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| Abstract: | Objective To identify the putative interaction of novel gene Ang Re m104 and glucocorticoid receptor AF-1 specific elongation factor in mammalian c ells. Methods The human embryonic kidney (HEK 293T) cells were transformed with two recombined plasmids, AngRem104-pcDNA3 1/V5-His and GR-EF-pCMV/c-myc. M ouse anti human V5 monoclonal antibody and mouse anti human c-myc monoclonal an tibody were respectively used in immunoprecipitation and immunoblot analysis. Re sults The AngRem104-V5 and the GR-EF-c-myc fusion protein were detected resp ectively in the immunoprecipitation by anti c-myc and anti V5 antibody. Conclus ion The novel gene AngRem104 may interact with glucocorticoid receptor AF-1 spe cific elongation factor in mammalian cells. |
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| Keywords: | Glucocorticoid receptors Elongation factor Immunoprecipitation AngRem104 |
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