| 自分泌IL-4对K562细胞诱导的CTL杀伤活性的影响 |
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| 引用本文： | 赵晓东,李成荣,杨锡强,蒋利萍,王莉佳. 自分泌IL-4对K562细胞诱导的CTL杀伤活性的影响[J]. 重庆医科大学学报, 2000, 25(1): 1-3 |
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| 作者姓名： | 赵晓东 李成荣 杨锡强 蒋利萍 王莉佳 |
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| 作者单位： | 重庆医科大学儿科学院免疫室 400014 |
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| 基金项目： | 国家自然科学基金;39470733; |
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| 摘 要： | 探讨ＩＬ－４基因修饰对Ｋ５６２细胞诱导的肿瘤特异性ＣＴＬ杀伤活性的促进作用及可能机理。采用逆转录病毒体ＬＸＳＮ将ＩＬ－４ ｃＤＮＡ导入Ｋ５６２细胞，经Ｇ４１８筛选后获得表达ＩＬ－４蛋白的Ｋ５６２克隆。以野生型和载体修饰的相应细胞为对照，检测了ＩＬ－４基因修饰Ｋ５６２细胞诱导的Ｔ细胞增殖反应，用＾５１铬（＾５１Ｃｒ）释放法检测ＣＴＬ杀伤活性。结果发现ＩＬ－４基因修饰的Ｋ５６２细胞诱导的Ｔ细胞增殖反应
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| 关 键 词： | IL－4 基因治疗 急性白血性 CTL |
| 文章编号： | 0253-3626(2000)01-0001-03 |
| 修稿时间： | 1999-04-26 |
Effect of autocrine IL-4 on killing activity of CTL induced by K562 |
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| ZHAO Xiao-dong,et al. Effect of autocrine IL-4 on killing activity of CTL induced by K562[J]. Journal of Chongqing Medical University, 2000, 25(1): 1-3 |
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| Authors: | ZHAO Xiao-dong et al |
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| Abstract: | To observe the effect and possible mechanism of IL-4 gene modification on killing activity of K562 -specific CTL, IL-4 cDNA was transduced into K562 cell via the retroviral vector-mediated gene transfer method. G418-resistant clones were obtained and expression of IL-4 of IL-4 gene-engineered and parental K562 cells was determined. Meanwhile, proliferation of normal PBMC cells were assayed by 3H-TdR incorporation and the 51Cr release test, respectively. The results showed that proliferation of normal PBMC and killing activity of CTL induced by IL-4 positive K562 cells were greatly increased. It was concluded that IL-4 gene modification is capable of upregulating the killing activity of K562-specific CTL by possibly enhancing the T lylnphocyte function. |
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| Keywords: | Interleukin-4 Gene therapy Leukemia |
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