维生素D3抑制角质形成细胞CXCR2的表达

目的 观察维生素D3对角质形成细胞表面表达CXCR2的影响,探讨其在治疗银屑病中的作用机制。方法 用不同浓度的维生素D3处理人角质形成细胞株HaCaT,48h后通过四甲基偶氮唑盐(MTT)法检测了维生素D3抑制HaCaT增殖的有效剂量;通过流式细胞仪(FACS)分析维生素D3处理的HaCaT表达趋化因子受体CXCR2的情况。结果 HaCaT经维生素D3在8.0×10^-8mol/L～5.12×10^-6mol/L浓度范围内处理48h后的A值较未处理组明显降低(P<0.05).HaCaT在5.12×10^-6mol/L维生素D3处理48h后CXCR2的表达明显低于正常对照组。结论 维生素D3治疗银屑病的作用机制部分是通过抑制角质形成细胞表达CXCR2,从而达到抑制角质形成细胞增殖的目的。

The Inhibitory Effect of Vit D3 on the Expression of CXCR2 on Keratinocytes

Objective To detect the expression of CXCR2on p soriatic lesional keratinocytes an d to in-vestigate the mechanism of Vit D3in t he treatment of psoriasis.Methods The proliferative capacity of human keratinocyte cell line,HaCaT,trea ted with Vit D3was detected by MTT assays.The expression of CXCR2on HaCaT treated with Vit D3was assayed by FACS calibur.Results The Vit D3at proper concentrations c ould inhibit the proliferation of HaCaT and down-regulate the CXCR2expression on the cells.Conclusion One of the mechanisms for Vit D3in the treatment of psoriasis might be the inhibition of CXCR2expression on keratinocytes,which con-tributes to inhibit the over-proliferation of keratinocytes in psoriasis.