| RNA干扰中小干扰RNA的设计原则 |
| |
| 引用本文： | 王志勇,吕延成. RNA干扰中小干扰RNA的设计原则[J]. 医学综述, 2010, 16(5) |
| |
| 作者姓名： | 王志勇 吕延成 |
| |
| 作者单位： | 遵义医学院珠海校区免疫学教研室,广东,珠海,519041 |
| |
| 摘 要： | RNA干扰(RNAi)是由内源性或外源性双链RNA介导的一种高度保守的序列特异性基因表达调控机制,主要通过小干扰RNA(siRNA)和微小RNA发挥作用。由于其具有高效、高特异性、作用稳定等特点,已被广泛地应用于基础实验和疾病治疗的研究中。在进行siRNA介导的RNAi实验时,如何设计高效、特异性抑制靶mRNA的siRNA是实验成败的关键。
|
| 关 键 词： | RNA干扰 小干扰RNA 设计原则 |
The Research Progress of the Designing Principles for Small Interfering RNA in RNAi Interference |
| |
| WANG Zhi-yong,LV Yan-cheng.. The Research Progress of the Designing Principles for Small Interfering RNA in RNAi Interference[J]. Medical Recapitulate, 2010, 16(5) |
| |
| Authors: | WANG Zhi-yong LV Yan-cheng. |
| |
| Affiliation: | WANG Zhi-yong,LV Yan-cheng.(Department of Immunity,Zhuhai Campus of Zunyi Medical College,Zhuhai 519041,China) |
| |
| Abstract: | RNAi interference(RNAi)is a highly conserved and sequence-specific regulatory mechanism of gene expression induced by exogenous or endogenous,double-stranded RNA,primarily by small interfering RNA(siRNA)and microRNA(miRNA).Due to its high efficiency,high specificity and good stability,RNAi has been widely used in the research of basic experiment and treatment of disease.During the RNAi experiment induced by siRNA,how to design the efficient and specific siRNA is the key of a successful experiment. |
| |
| Keywords: | RNA interference Small interfering RNA Designing principles |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
