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| 移植物抗宿主病小鼠肝脏及异体CD8~+T细胞趋化因子和趋化因子受体表达分析 | |
| 引用本文： | 曹琦,何珊,邱玉华,马泓冰,周同,沈天伟,陈罗刚,张雁云.移植物抗宿主病小鼠肝脏及异体CD8~+T细胞趋化因子和趋化因子受体表达分析[J].现代免疫学,2007,27(5):397-402. |
| 作者姓名： | 曹琦 何珊 邱玉华 马泓冰 周同 沈天伟 陈罗刚 张雁云 |
| 作者单位： | 1. 上海交通大学医学院,上海市免疫学研究所,上海,200025 2. 中国科学院上海生命科学研究院/上海交通大学医学院健康科学研究所,上海,200025 3. 苏州大学医学院,苏州,215007 4. 上海交通大学医学院附属瑞金医院,上海,200025 |
| 基金项目： | 国家自然科学基金;上海市重点学科建设项目 |
| 摘 要： | 为探讨趋化因子及其受体介导的效应性细胞迁移在移植物抗宿主病(graft-versus-host disease,GVHD)发生发展中的重要作用,采用次要组织相容性抗原异配的GVHD小鼠模型,应用流式细胞分选术(FACS)、实时荧光定量聚合酶链反应等方法,分析GVHD靶器官和异体CD8+T细胞趋化因子及受体的表达。肝脏高表达干扰素诱导蛋白-10(IP-10)、干扰素诱导的T细胞趋化因子(ITAC)、γ干扰素诱生单核因子(MIG)、巨噬细胞炎症蛋白(MIP)-1α/β等趋化因子。异体CD8+T细胞上则高表达CXC趋化因子受体(CXCR)3和CC趋化因子受体(CCR)5。随着肝脏趋化因子的表达增加,异体CD8+T细胞迁移浸润的数量也增高。因而趋化因子及异体CD8+T细胞上趋化因子受体的特异对应关系,促使大量异体CD8+T细胞迁移浸润并造成肝脏损伤。 |
| 关 键 词： | 趋化因子及其趋化因子受体 移植物抗宿主病 异体CD8+T细胞 |
| 文章编号： | 1000-2478（2007）05-0397-06 |
| 修稿时间： | 2007-04-06 |
Chemokines and chemokine receptors expressed by the liver and allo-reactive CD8+ T cells in graft-versus-host disease mouse |
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| CAO Qi,HE Shan,QIU Yu-hua,MA Hong-bin,ZHOU Tong,SHEN Tian-wei,CHEN Luo-gang,ZHANG Yan-yun.Chemokines and chemokine receptors expressed by the liver and allo-reactive CD8+ T cells in graft-versus-host disease mouse[J].Current Immunology,2007,27(5):397-402. | |
| Authors: | CAO Qi HE Shan QIU Yu-hua MA Hong-bin ZHOU Tong SHEN Tian-wei CHEN Luo-gang ZHANG Yan-yun |
| Institution: | Shanghai Institute of Immunology, Shanghai Jiaotong University, School of Medicine, Shanghai 200025, China; Shanghai Institutes for Biological Sciences/Shanghai J iaotong University, School of Medicine, Institute of Health Sciences, Shanghai 200025, China; Soochow University, School of Medicine, Suzhou 215007, China; Shanghai Jiaotong University, Affiliated Ruijin Hospital, Shanghai 200025, China |
| Abstract: | To investigate the important roles of chemokines and chemokine receptors in regulating the migration of effect cells in graft-versus-host disease(GVHD),a murine GVHD model to analysis chemokine and chemokine receptor expression in the liver and the allo-CD8+ T cell was used.By means of flow cytometry analysis(FACS) and real-time PCR,we found that liver of GVHD expressed high level of interferon inducible protein-10(IP-10),interferon-inducible T cell alpha chemoattractant(ITAC),monokine induced by IFN-γ(MIG),and macrophage inflammatory protein(MIP)-1α/β.The infiltrated allo-CD8+ T cells,accordingly,expressed high CXC chemokine receptor 3(CXCR3) and CC chemokine receptor 5(CCR5).The number of allo-CD8+ T cells in liver increased along with the up-regulated expression of chemokines,so the relative specific relation of the allo-CD8+ T cells and the target tissue of GVHD encouraged the migration and infiltration of allo-CD8+ T cells and caused tissue damage. |
| Keywords: | chemokine and chemokine receptor graftversus-host disease(GVHD) allo-CD8 T cell |
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