毛茛总苷对离体蛙心和大鼠胸主动脉环张力的作用研究

目的:研究毛茛总苷对离体蛙心和大鼠胸主动脉环张力的影响。方法:制备离体蛙心,观察毛茛总苷对蛙心基础张力和异丙肾上腺素诱导的心脏收缩力的影响;制备大鼠离体胸主动脉环,观察毛茛总苷对主动脉基础张力、去甲肾上腺素诱导主动脉环收缩和对异丙肾上腺素诱导主动脉环舒张的影响。结果:1μg/mL和10μg/mL毛茛总苷可以降低离体蛙心的基础张力,并具有抑制异丙肾上腺素诱导蛙心收缩作用,但对心率没有影响;1mg/mL和2mg/mL的毛茛总苷对主动脉环的基础张力和对异丙肾上腺素诱导的主动脉环舒张均没有影响;2mg/mL的毛茛总苷能够明显拮抗由去甲肾上腺素诱导的主动脉环收缩作用,与生理盐水组相比有明显的差异(P<0.01),与酚妥拉明组相比没有明显差异(P>0.05);1mg/mL的毛茛总苷也能拮抗去甲肾上腺素诱导的血管环收缩作用,但与生理盐水组相比无明显的差异(P>0.05)。结论:毛茛总苷具有抑制异丙肾上腺素诱导的蛙心收缩作用;毛茛总苷具有抑制去甲肾上腺素诱导的大鼠胸主动脉环收缩作用,但对主动脉环的基础张力和异丙肾上腺素诱导的动脉舒张均无影响。

Effect of Total Glycosides of Ranunculus Japonicus (TGOR) on Tension of Frog Heart and Rat Chest Aortic Ring in Vitro

Objective:To study effect of total glycosides of Ranunculus japonicus(TGOR)on tension of frog heart and rat chest aortic ring in vitro.Methods:Isolated frog hearts were prepared to study effect of TGOR on elementary contraction force and isoprenaline-induced myocardial contractility.Effect of TGOR on elementary tension,tension of of isolated rat thoracic aortic ring in response to arterenol or isoprenaline were furtherly observed.Results:Show that 1g/mL and 10g/mL TGOR was able to underlied elementary contraction force of isolated flog hearts and prevented myocardial contractility from rising in response to isoprenaline,but did not affect heart rate.1mg/mL or 2mg/mL TGOR had no effect on the elementary tension of arterial circle or isoprenaline-induced vasorelaxation.2mg/mL TGOR obviously inhibited noradrenaline-induced vasoconstriction,vs saline(P<0.01),vs phentolamine(P>0.05);1mg/mL TGOR was also able to inhabit noradrenaline-induced vasoconstriction,P>0.05,vs saline.Conclusion:TGOR inhibited effect of isoprenaline-induced frog heart contraction.TGOR underlied effect of noradrenaline-induced rat chest arterial circle and had not affected elementary tension and isoprenaline-induced vasorelaxation.