大鼠脑缺血预处理对额叶神经细胞凋亡和P53蛋白表达的影响

目的探讨大鼠短暂性全脑缺血预处理对再次脑缺血额叶神经细胞凋亡和P53蛋白表达的影响.方法采用改良的Pulsinelli 4血管阻断(4VO)方法,建立SD大鼠急性全脑缺血及预处理模型.雄性SD大鼠随机分为三组预处理对照组,给予3min全脑缺血;预处理缺血组,先给予3min全脑缺血,48h后在给予全脑缺血15min;缺血组,仅给予全脑缺血15min.采用TUNEL方法观察额叶神经细胞凋亡,SP免疫组化方法检测P53蛋白的表达.结果预处理对照组未见TUNEL阳性细胞,仅见个别P53蛋白阳性细胞.预处理缺血组与缺血组相比,再灌注后48h、72h及7d额叶TUNEL阳性细胞数显著减少(P《0.01).预处理缺血组与缺血组相比,再灌注后48h、72h及7d额叶P53阳性细胞数显著减少(P《0.01).结论全脑缺血15 min后额叶神经细胞凋亡和P53蛋白表达增多.全脑缺血预处理能减少额叶缺血再灌注后神经细胞凋亡和P53蛋白的表达.

Effects of ischemic preconditioning on neuronal cell apoptosis and P53 protein expression in frontal lobe in rats

Objective To investigate the effects of ischemic preconditioning on neuronal cell apoptosis and P53 protein expression of frontal lobe in rats . Methods The acute global cerebral ischemia and ischemic preconditioning was produced by four - vessel occlusion as described by Pulsinelli in Spraque - daewley rats. The rats were randomly divided into three groups of 7 animals, A: ischemic preconditioning group; B: ischemia - reperfussion group (I/P); C: ischemic preconditioning + I/P group. We used TUNEL staining to label the neuronal apoptosis and immunohistochemistry to detect the expression of P53 protein. Results The number of TUNEL positive cells in ischemic preconditioning + I/P group decreased greatly compared with ischemic group (p < 0.01) . The P53 protein expression in ischemic preconditioning group was markedly diminished than that in ischemic - reperfussion group (p < 0.01) . Conclusion The global ischemic - reperfussion could induce the neuronal cell apoptosis and P53 protein expression of frontal lobe in rats. The global ischemic preconditioning could reduce P53 protein expression and suppress the neuronal apoptosis of the frontal lobe.