| HPLC法同时测定甘草指纹图谱暨甘草苷、甘草酸含量 |
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| 引用本文： | 段天璇,于密密,刘春生,马长华,王文全,魏胜利. HPLC法同时测定甘草指纹图谱暨甘草苷、甘草酸含量[J]. 中成药, 2006, 28(2): 161-165 |
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| 作者姓名： | 段天璇 于密密 刘春生 马长华 王文全 魏胜利 |
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| 作者单位： | 北京中医药大学中药学院,北京,100102 |
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| 基金项目： | 国家自然科学基金资助项目(30400588) |
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| 摘 要： | 目的:建立甘草甲醇提取物的HPLC指纹图谱测定方法,并同时对其中甘草苷(liqu iritin,L)、甘草酸(glycyrrh izicac id,GA)的含量进行测定;方法:筛选适当提取方法,采用C18反相色谱柱,0.1%磷酸水-乙腈梯度洗脱,对不同色谱峰采用不同紫外波长检测;结果:测定了不同产地野生甘草及同一产地不同年限和部位栽培甘草的指纹图谱和甘草酸、甘草苷含量;结论:方法准确、稳定、可靠,可用于甘草的质量研究和评价。
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| 关 键 词： | 甘草 HPLC指纹图谱 甘草酸 甘草苷 |
| 文章编号： | 1001-1528（2006）02-0161-03 |
| 收稿时间： | 2005-07-01 |
| 修稿时间： | 2005-07-01 |
Simultaneous determination of glycyrrhizic acid, liquiritin and fingerprint of licorice by RP-HPLC |
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| DUAN Tian-xuan,YU Mi-mi,LIU Chun-sheng,MA Chang-hua,WANG Wen-quan,WEI Sheng-li. Simultaneous determination of glycyrrhizic acid, liquiritin and fingerprint of licorice by RP-HPLC[J]. Chinese Traditional Patent Medicine, 2006, 28(2): 161-165 |
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| Authors: | DUAN Tian-xuan YU Mi-mi LIU Chun-sheng MA Chang-hua WANG Wen-quan WEI Sheng-li |
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| Affiliation: | College of Chinese Pharmacy, Beijing University of Traditional Chinese Medicine, Bejing 100102, China |
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| Abstract: | AIM: To develop a HPLC fingerprint and quantitative determination of glycyrrhizic acid(GA)and liquiritin(L)simultaneously for licorice of various species,parts and origins. METHODS: The 67% metanol extracts of licorice powder were analyzed by RP-HPLC on an ODS column,using a gradient elution with a water((contains) 0.1%phosphoric acid)-acetonitrile mixture.The eluate was monitored by UV absorption at 248,275 and 360 nm. RESULTS: Fifteen common peaks characteristic of licorice roots were found as well as GA and L were determined for 16 samples. CONCLUSION: The method provides some useful information about the chemical constituents of licorice and covers the chemical contents prescribed by China pharmacopoeia(2005) |
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| Keywords: | licorice HPLC fingerprint glycyrrhizic acid liquiritin |
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